Efficient metal-specific transcription activation by Drosophila MTF-1 requires conserved cysteine residues in the carboxy-terminal domain.
Efficient metal-specific transcription activation by Drosophila MTF-1 requires conserved cysteine residues in the carboxy-terminal domain.
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果蝇 MTF-1 的有效金属特异性转录激活需要羧基末端结构域中的保守半胱氨酸残基。
DOI：
10.1016/j.bbagrm.2012.03.005
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Marr,MichaelT
中科院分区：
文献类型：
--
作者：
Marr,SharonK;Pennington,KatieL;Marr,MichaelT
MTF-1 is a sequence-specific DNA binding protein that activates the transcription of metal responsive genes. The extent of activation is dependent on the nature of the metal challenge. Here we identify separate regions within the Drosophila MTF-1 (dMTF-1) protein that are required for efficient copper- versus cadmium-induced transcription. dMTF-1 contains a number of potential metal binding regions that might allow metal discrimination including a DNA binding domain containing six zinc fingers and a highly conserved cysteine-rich C-terminus. We find that four of the zinc fingers in the DNA binding domain are essential for function but the DNA binding domain does not contribute to the metal discrimination by dMTF-1. We find that the conserved C-terminus of the cysteine-rich domain provides cadmium specificity while copper specificity maps to the previously described copper-binding region (Chen et al.). In addition, both metal specific domains are autorepressive in the absence of metal and contribute to the low level of basal transcription from metal inducible promoters.
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影响因子：
2.9
作者：
Chen, XH;Chu, MH;Giedroc, DP
通讯作者：
Giedroc, DP
人类金属硫蛋白 (MT) 基因的调节。
DOI：
10.1016/s0021-9258(18)69121-5
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Sadhu;L. Gedamu
通讯作者：
L. Gedamu
DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
Clemens, JC;Worby, CA;Dixon, JE
通讯作者：
Dixon, JE
影响因子：
2.9
作者：
Chen, XH;Agarwal, A;Giedroc, DP
通讯作者：
Giedroc, DP
影响因子：
3.9
作者：
Fabisiak, JP;Tyurin, VA;Kagan, VE
通讯作者：
Kagan, VE