Evaluation of the strains of Acinetobacter and Enterobacter as potential biocontrol agents against Ralstonia wilt of tomato
Evaluation of the strains of Acinetobacter and Enterobacter as potential biocontrol agents against Ralstonia wilt of tomato
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DOI：
10.1016/j.biocontrol.2008.11.004
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发表时间：
2009-03-01
影响因子：
4.2
通讯作者：
Guo, Jian-Hua
中科院分区：
文献类型：
--
作者：
Xue, Qing-Yun;Chen, Yu;Guo, Jian-Hua
番茄青枯病（Ralstonia solanacearum）对中国南方的番茄造成相当大的损害。生物防治是减少该病害发生率和产量损失的最有前途的方法之一。基于对青枯菌和三种土传真菌病原体的拮抗活性以及温室中的生防效果，从 14 种候选菌株中选择了两种细菌菌株 Xa6（不动杆菌属）和 Xy3（肠杆菌属）作为潜在的生防剂。为了寻找合适的拮抗剂接种方法，我们从根系能力、生防效果以及温室条件下促进植物生长的效果等方面对浸根法和浸土法进行了比较。淋灌处理具有较高的生防效果和植物产量，且更易于大田操作。进行了田间试验以进一步评估这两种拮抗菌株。在温室和大田试验中，菌株Xy3对青枯病的防治效果均优于Xa6，同时Xa6引起了更高的生物量或产量增加。根据两次田间试验处理后第75天的记录，两次田间试验中Xy3的生防效果约为65%，Xa6引起的产量增加分别为32.4%和40.7%。这是不动杆菌属的第一份报告。菌株用作对抗番茄 Ralstonia 枯萎病的 BCA。 (C) 2008 Elsevier Inc. 保留所有权利。
Bacterial wilt (Ralstonia solanacearum) of tomato, Lycopersicon esculentum, causes a considerable amount of damage to tomato in Southern China. Biological control is one of the more promising approaches to reduce the disease incidence and yield losses caused by this disease. Based on antagonistic activity against R. solanacearum and three soil-borne fungal pathogens as well as biocontrol efficacy in the greenhouse, two bacterial strains Xa6 (Acinetobacter sp.) and Xy3 (Enterobacter sp.) were selected out of fourteen candidates as potential biocontrol agents. In order to find a suitable antagonist inoculation method, we compared the methods of root-dipping with soil-drenching in the aspects including rhizocompetence, biocontrol efficacy, and effect of promoting plant growth under greenhouse conditions. The drenching treatment resulted in a higher biocontrol efficacy and plant-yield increase, and this method was also easier to operate in the field on a large scale. Field trials were conducted for further evaluation of these two antagonistic strains. In both greenhouse and field experiments, the strain Xy3 had a better control effect against bacterial wilt than Xa6 did, while Xa6 caused higher biomass or yield increases. As recorded on the 75th day after treatment in two field experiments, biocontrol efficacy of Xy3 was about 65% in both field trials, and the yield increases caused by Xa6 were 32.4 and 40.7%, respectively, in the two trials. This is the first report of an Acinetobacter sp. strain used as a BCA against Ralstonia wilt of tomato. (C) 2008 Elsevier Inc. All rights reserved.