Physical mapping of black spot disease resistance/susceptibility-related genome regions in Japanese pear (Pyrus pyrifolia) by BAC-FISH.
Physical mapping of black spot disease resistance/susceptibility-related genome regions in Japanese pear (Pyrus pyrifolia) by BAC-FISH.
复制标题
通过 BAC-FISH 对日本梨 (Pyruspyrifolia) 黑斑病抗性/易感性相关基因组区域进行物理定位。
DOI：
10.1270/jsbbs.15085
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发表时间：
2016-06
期刊：
影响因子：
2.4
通讯作者：
Yamamoto T
中科院分区：
文献类型：
--
作者：
Yamamoto M;Terakami S;Takada N;Yamamoto T
黑斑病是日本梨栽培中危害最大的病害之一，是由日本梨互交花孢病菌引起的黑斑病。本研究利用BAC克隆（BAC- fish）对日本梨（Pyrus pyrifolia (Burm)）黑斑病抗性/敏感性相关基因组区域的位置进行了荧光原位杂交研究。f。)Nakai)染色体。以“Osa Gold”自花授粉幼苗的根尖为材料。采用酶浸风干法制备染色体样品。靠近黑斑病相关基因的BAC克隆被标记为FISH分析的探针。在两个中等大小染色体的端粒位置检测到黑斑病相关的基因组区域。这两个位点和6个端粒18S-5.8S-25S rDNA位点分别位于不同的染色体上。通过BAC-FISH方法获得的梨染色体上有用基因物理定位的有效性得到了明确的证明。
Black spot disease, caused by Alternaria alternata Japanese pear pathotype, is one of the most harmful diseases in Japanese pear cultivation. In the present study, the locations of black spot disease resistance/susceptibility-related genome regions were studied by fluorescence in situ hybridization using BAC clone (BAC-FISH) on Japanese pear (Pyrus pyrifolia (Burm. f.) Nakai) chromosomes. Root tips of self-pollinated seedlings of ‘Osa Gold’ were used as materials. Chromosome samples were prepared by the enzymatic maceration and air-drying method. The BAC clone adjacent to the black spot disease-related gene was labeled as a probe for FISH analysis. Black spot disease-related genome regions were detected in telomeric positions of two medium size chromosomes. These two sites and six telomeric 18S-5.8S-25S rDNA sites were located on different chromosomes as determined from the results of multi-color FISH. The effectiveness of the physical mapping of useful genes on pear chromosomes achieved by the BAC-FISH method was unequivocally demonstrated.