The Passive Permeability of the Red Blood Cell to Cations
The Passive Permeability of the Red Blood Cell to Cations
复制标题
红细胞对阳离子的被动渗透性
DOI：
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发表时间：
1966
期刊：
影响因子：
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通讯作者：
A. Rothstein
中科院分区：
文献类型：
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作者：
P. Lacelle;A. Rothstein
在稳态条件下测量了悬浮在等渗蔗糖加低浓度盐中的人红细胞的盐流出。流出量和盐浓度的对数之间的关系可以通过具有尖锐拐点的两条直线拟合，在低于0.2 mM NaCl的浓度下出现更陡的斜率。外排速率的决定因素是离子强度，而不是特定的一价阳离子或阴离子，并且影响是完全可逆的。随着温度的升高，离子强度降低的影响更加明显，拐点向更高的盐浓度移动。在给定的离子强度下，pH值的增加导致流出量增加，但在低离子强度下，pH效应的大小很小。在给定的pH值下，数据可以通过简化形式的Goldman方程拟合，这表明随着离子强度的降低，渗透率保持恒定，直到达到拐点。在该离子强度下，发生向新的渗透性状态的急剧可逆转变。渗透性随外部pH值而非内部pH值的增加而增加。
The efflux of salt from human red blood cells suspended in isotonic sucrose plus low concentrations of salt, was measured under steady-state conditions. The relationship between the efflux and the log of the salt concentration can be fitted by two straight lines with a sharp inflection point, the steeper slope occurring at concentrations below 0.2 mM NaCl. The determining factor in the rate of efflux is the ionic strength rather than the specific monovalent cations or anions and the effects are completely reversible. With an increase in temperature, the effects of reduced ionic strength are more pronounced and the inflection point is shifted toward higher salt concentrations. An increase in pH leads to an increased efflux at a given ionic strength, but the size of the pH effect is small at low ionic strength. At a given pH, the data can be fitted by a simplified form of the Goldman equation suggesting that with reduction in ionic strength, the permeability remains constant until the inflection point is reached. At that ionic strength, a sharp reversible transition to a new permeability state occurs. The permeability increases with an increase in the external but not the internal pH.