Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis
Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis
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DOI：
10.1111/nph.18385
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发表时间：
2022-08-03
期刊：
影响因子：
9.4
通讯作者：
Yano，Kentaro
中科院分区：
文献类型：
--
作者：
Koshimizu，Shizuka;Minamino，Naoki;Yano，Kentaro
陆地植物精子通常具有典型的结构，如花键（由微管阵列组成）、多层结构（最上层是花键的连续体）和多个鞭毛。然而，支持精子发生的分子机制仍有待阐明。通过结合多种方法和组学数据的计算分析，我们成功地鉴定了参与精子发生的候选基因，深度分化的bld10s。然后，我们检测了bld10在肝细胞marchantia多态性和大多数spyscomitrium patens中的功能。mpbld10和ppbld10是正常基底体（BB）和鞭毛形成所必需的。mpbld10突变体在精子形成过程中表现出细胞质和细胞核重塑的缺陷，因此mpbld10可能参与了精子发生过程中细胞质的染色质重组和消除。我们在多种链藻中鉴定了MpBLD10和PpBLD10的同源物，发现MpBLD10和PpBLD10与在BB组装中起作用的BLD10/CEP135家族蛋白同源。然而，bld10在陆地植物中进化得特别快，MpBLD10可能通过快速的分子进化在精子形成中获得了额外的功能。
Land plant spermatozoids commonly possess characteristic structures such as the spline, which consists of a microtubule array, the multilayered structure (MLS) in which the uppermost layer is a continuum of the spline, and multiple flagella. However, the molecular mechanisms underpinning spermatogenesis remain to be elucidated.We successfully identified candidate genes involved in spermatogenesis, deeply divergentBLD10s, by computational analyses combining multiple methods and omics data. We then examined the functions ofBLD10s in the liverwortMarchantia polymorphaand the mossPhyscomitrium patens.MpBLD10and PpBLD10are required for normal basal body (BB) and flagella formation. Mpbld10mutants exhibited defects in remodeling of the cytoplasm and nucleus during spermatozoid formation, and thus MpBLD10should be involved in chromatin reorganization and elimination of the cytoplasm during spermiogenesis.We identified orthologs of MpBLD10 and PpBLD10 in diverse Streptophyta and found that MpBLD10 and PpBLD10 are orthologous to BLD10/CEP135 family proteins, which function in BB assembly. However, BLD10s evolved especially quickly in land plants and MpBLD10 might have acquired additional functions in spermatozoid formation through rapid molecular evolution.