Integrated analysis of miRNA and mRNA expression profiling in bovine endometrial cells in response to lipopolysaccharide-stimulation
Integrated analysis of miRNA and mRNA expression profiling in bovine endometrial cells in response to lipopolysaccharide-stimulation
复制标题
牛子宫内膜细胞响应脂多糖刺激的 miRNA 和 mRNA 表达谱综合分析
DOI：
10.1016/j.micpath.2017.11.053
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发表时间：
2018-01-01
影响因子：
3.8
通讯作者：
Lu, Wenfa
中科院分区：
文献类型：
--
作者：
Wang, Jun;Yan, Xiaoxiao;Lu, Wenfa
microRNA（miRNA）在基因表达调控中发挥着重要作用，是研究基因表达调控机制的重要资源。脂多糖（LPS）诱导牛子宫内膜细胞炎症反应的分子机制尚未得到广泛研究。在本研究中，通过RNA-Seq（RNA测序）评估了用1 pg/mL LPS处理24 h的牛子宫内膜细胞的miRNA和mRNA表达谱。结果显示，LPS诱导了20个（11个上调，9个下调）牛子宫内膜细胞差异表达的miRNAs和108个（90个上调，18个下调）差异表达的mRNA。通过定量实时PCR（qRT-PCR）评估5种mRNA和4种miRNA的结果，以验证RNA-seq数据的可靠性。整合miRNA和mRNA表达谱分析显示116个miRNA靶基因对。GO和KEGG通路分析差异表达的miRNAs和靶基因预测差异表达的miRNAs在LPS诱导的牛子宫内膜细胞炎症反应中的可能作用。通过qRT-PCR测量三个miRNA-靶基因对的表达来验证miRNA和mRNA数据的整合分析的可靠性。我们的研究结果提高了对miRNA参与LPS诱导的牛子宫内膜细胞炎症反应的作用的理解。
MicroRNAs (miRNA) play an important role in regulating gene expression, making them important resources for exploring molecular mechanisms. Molecular mechanisms involved in the inflammatory responses of bovine endometrial cells induced by lipopolysaccharide (LPS) have not been widely studied. In the present study, miRNA and mRNA expression profiling of bovine endometrial cells treated with 1 pg/mL LPS for 24 h were evaluated by RNA-Seq (RNA-sequencing). The results showed that LPS induced 20 (11 up- and 9 down-regulated) differentially-expressed miRNAs and 108 (90 up- and 18 down-regulated) differentially-expressed mRNAs of bovine endometrial cells. The results for 5 mRNAs and 4 miRNAs were evaluated by quantitative real-time PCR (qRT-PCR) to validate the reliability of the RNA-seq data. Integrating analysis of the miRNA and mRNA expression profiles revealed 116 miRNA-target gene pairs. GO and KEGG pathway analysis of differentially expressed miRNAs and target genes predicted the likely roles of differentially expressed miRNAs in inflammatory responses in bovine endometrial cells induced by LPS. The reliability of the integrating analysis of the miRNA and mRNA data were validated by measuring the expression of three miRNA-target gene pairs by qRT-PCR. Our results improve the understanding of the role of miRNA involvement in inflammatory response of bovine endometrial cells induced by LPS.