Serum leptin levels negatively correlate with trabecular bone mineral density in high-fat diet-induced obesity mice.
Serum leptin levels negatively correlate with trabecular bone mineral density in high-fat diet-induced obesity mice.
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发表时间：
2012-06
影响因子：
1.9
通讯作者：
Y. Fujita;K. Watanabe;K. Maki
中科院分区：
文献类型：
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作者：
Y. Fujita;K. Watanabe;K. Maki
目的探讨饮食诱导肥胖对生长期小鼠胫骨近端骨组织数量和质量的影响，以及血清总胆固醇、瘦素、脂联素水平与骨小梁和皮质骨矿物质参数的关系。方法6周龄雄性C57BL/6J小鼠随机分为对照组和高脂饲料组，每组10只。治疗4、8、12周后，用外周定量计算机断层扫描(PQCT)、显微计算机断层扫描和组织形态计量学分析骨的数量和质量。结果早期骨小梁密度降低，脂肪细胞增多，骨小梁恶化。相反，尽管肥胖小鼠的皮质骨形成比对照组小鼠慢，但骨膜表面的骨形成随着年龄的增长而增加。在饮食诱导肥胖的小鼠中，血清瘦素水平与骨小梁密度相关，但与皮质骨密度无关，而脂联素和总胆固醇水平与骨量均不相关。结论我们得出结论，在小鼠中，这两个部位的骨丢失受到不同的调节。此外，我们证明血清瘦素可能是与饮食诱导肥胖相关的骨质疏松症风险的有用指标。
OBJECTIVES This study evaluated the influence of diet-induced obesity on bone tissue quantity and quality in the proximal tibiae of growing mice and also examined the relationships between the serum total cholesterol, leptin, and adiponectin levels and trabecular and cortical bone mineral parameters. METHODS Six-week-old male C57BL/6J mice were divided into two groups; one received a control diet, and the other received a high-fat-diet. After treatment for 4, 8, or 12 weeks, the bone quantity and quality were analyzed using peripheral quantitative computed tomography (pQCT), micro-computed tomography and histomorphometry. RESULTS In the early stages, trabecular bone density decreased with an increase in the number of adipocytes and the deterioration of trabeculae. In contrast, although cortical bone formation was slower in obese mice compared with control mice, bone formation on the periosteal surface increased with age. Serum leptin levels were correlated with trabecular, but not cortical bone density, whereas neither the adiponectin nor total cholesterol level was correlated with bone mass in mice with diet-induced obesity. CONCLUSIONS We conclude that bone loss at these two sites is differentially regulated in mice. Furthermore, we demonstrate that serum leptin may be a useful indicator of risk for osteoporosis associated with diet-induced obesity.