Volatile organic compounds of Bacillus cereus strain Bc-cm103 exhibit fumigation activity against Meloidogyne incognita
Volatile organic compounds of Bacillus cereus strain Bc-cm103 exhibit fumigation activity against Meloidogyne incognita
复制标题
蜡样芽孢杆菌菌株 Bc-cm103 的挥发性有机化合物对南方根结线虫表现出熏蒸活性
DOI：
10.1094/pdis-04-20-0783-re
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发表时间：
2020
期刊：
影响因子：
4.5
通讯作者：
Mao Z.
中科院分区：
文献类型：
--
作者：
Yin N.;Liu R.;Zhao J.;Khan R.A.A.;Li Y.;Ling J.;Liu W.;Yang Y.;Xie B.;Mao Z.
蜡样芽孢杆菌Bc-cm 103菌株具有杀线虫活性，因此已被用作防治根结线虫南方根结线虫的生物防治剂。然而，它仍然是未知的，是否挥发性有机化合物（VOCs）产生的B。蜡状芽孢杆菌Bc-cm 103对该菌有较好的生防效果。无名氏因此，在本研究中，我们研究了Bc-cm 103 VOCs对M的活性。无名氏B.蜡状芽孢杆菌Bc-cm 103对M.无名氏在三室培养皿中对Bc-cm 103发酵产生的VOCs进行体外评价，发现M. 24 h和48 h时，incognitaJ 2的阳性率分别为90.8%和97.2%。此外，Bc-cm 103 VOCs抑制M.双层盆栽试验结果表明，黄瓜根上的根瘿减少46.1%。利用固相微萃取-气相色谱-质谱联用技术从Bc-cm 103菌株中鉴定出21种挥发性有机物，包括烷烃、烯烃、酯类和硫化物。其中二甲基二硫醚（30.63%）和硫代丁二酸甲酯（30.29%）具有较强的杀线虫活性。总之，这些结果表明，B.蜡状芽孢杆菌Bc-cm 103菌株对M.无名氏
Bacillus cereusstrain Bc-cm103 shows nematicidal activity and, therefore, has been used as a biological control agent to control the root-knot nematodeMeloidogyne incognita. However, it remains unknown whether volatile organic compounds (VOCs) produced byB. cereusstrain Bc-cm103 are effective in biocontrol againstM. incognita. Therefore, in this study, we investigated the activity of Bc-cm103 VOCs againstM. incognita. TheB. cereusstrain Bc-cm103 significantly repelled the second-stage juveniles (J2s) ofM. incognita. In vitro evaluation of VOCs produced by the fermentation of Bc-cm103 in a three-compartment Petri dish revealed the mortality rates ofM. incognitaJ2s as 90.8% at 24 h and 97.2% at 48 h. Additionally, evaluation of the ability of Bc-cm103 VOCs to suppressM. incognitainfection in a double-layered pot test showed that root galls on cucumber roots decreased by 46.1%. Furthermore, 21 VOCs were identified from strain Bc-cm103 by solid-phase microextraction gas chromatography–mass spectrometry, including alkanes, alkenes, esters, and sulfides. Among them, dimethyl disulfide (30.63%) and S-methyl ester butanethioic acid (30.29%) were reported to have strong nematicidal activity. Together, these results suggest thatB. cereusstrain Bc-cm103 exhibits fumigation activity againstM. incognita.