Expression of interleukin-6 in the cornea in response to infection with different strains of Pseudomonas aeruginosa
Expression of interleukin-6 in the cornea in response to infection with different strains of Pseudomonas aeruginosa
复制标题
DOI：
10.1128/iai.67.5.2497-2502.1999
复制
发表时间：
1999-05-01
影响因子：
3.1
通讯作者：
Husband, AJ
中科院分区：
文献类型：
--
作者：
Cole, N;Bao, SS;Husband, AJ
引起角膜炎的铜绿假单胞菌菌株可以是细胞毒性的(6206)，也可以是侵袭性的(6294)，而引起隐形眼镜引起的急性红眼的菌株(Pair1)已被证明两者都不是。应用原位杂交技术检测白介素6(IL-6)表达细胞在小鼠角膜中的定位和定位，以及不同菌株感染后表达的变化。图像分析法检测IL-6阳性细胞数，酶联免疫吸附试验检测IL-6蛋白水平。用绿脓杆菌6294、6206或Pair1(2×10(6)CFU)感染BALB/c小鼠，每隔24 h检测角膜组织中IL-6mRNA的表达，6294和6206株感染后角膜上皮细胞中IL-6mRNA的表达迅速升高，实验结束时浸润性炎症细胞也呈IL-6mRNA阳性表达，而Pair1株攻击的角膜仅在攻击后4h出现明显的IL-6mRNA表达上调。小鼠角膜中存在三种不同的IL-6mRNA表达模式以响应这三种眼部分离的铜绿假单胞菌，所获得的所有三种铜绿假单胞菌在角膜中的mRNA表达数据与全眼匀浆的IL-6蛋白分析具有很好的相关性。对这些菌株的细胞因子反应的差异与每种菌株相关的病理差异相关，并可能为制定改进眼部炎症管理的策略提供机会。
Strains of Pseudomonas aeruginosa causing keratitis can be either cytotoxic (6206) or invasive (6294), while a strain (Paer1) causing contact lens-induced acute red eye has been shown to be neither. In situ hybridization was used to examine the location and identity of cells expressing interleukin-6 (IL-6) mRNA in the murine cornea and changes in expression in response to infection with different strains of P. aerginosa. The number of IL-6-positive cells was determined by image analysis, IL-6 protein levels were measured by an enzyme-linked immunosorbent assay. BALB/c mice were challenged by use of the wounded-cornea model with P. aeruginosa 6294, 6206, or Paer1 (2 x 10(6) CFU), At time intervals up to 24 h, postchallenge corneal tissue was probed for IL-6 mRNA IL-6 mRNA expression was rapidly elevated in the epithelium in response to strains 6294 and 6206, At the conclusion of the experiments, infiltrating inflammatory cells also stained positively for IL-6 mRNA, In contrast, corneas challenged with strain Paer1 showed significant upregulation of IL-6 mRNA only at 4 h postchallenge. Three distinct patterns of IL-6 mRNA expression in the mouse cornea occur in response to these three ocular isolates of P, aeruginosa, The data obtained for mRNA expression in the cornea for all three strains of P, aeruginosa correlated well with IL-6 protein analysis of whole-eye homogenates. Differences in the cytokine responses to these strains correlate with differences in the pathology associated with each strain and may offer an opportunity to develop strategies for the improved management of ocular inflammation.