Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
复制标题
Xenopus U3 snRNA 的核苷酸序列测定和二级结构。
DOI：
10.1093/nar/16.5.2127
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发表时间：
1988
影响因子：
14.9
通讯作者：
Gerbi,SA
中科院分区：
文献类型：
--
作者：
Jeppesen,C;Stebbins-Boaz,B;Gerbi,SA
通过结合RNA测序和构建cDNA克隆，然后进行DNA测序，我们确定了爪蟾和北极爪蟾中U3 snRNA的初级核苷酸序列，该分子的长度为219个核苷酸。非洲爪蟾序列与其他生物体的 U3 snRNA 序列的比对揭示了三个进化保守的区块。我们使用单链特异性化学试剂探测了完整非洲爪蟾核中 U3 snRNA 的二级结构；使用引物延伸来绘制化学修饰的位置图。这三个保守序列块位于单链区域内，因此可以与其他分子相互作用。根据这些数据讨论了 U3 snRNA 功能的模型。
Using a combination of RNA sequencing and construction of cDNA clones followed by DNA sequencing, we have determined the primary nucleotide sequence of U3 snRNA inXenopuslaevisandXenopusborealis This molecule has a length of 219 nucleotides. Alignment of theXenopussequences with U3 snRNA sequences from other organisms reveals three evolutionarily conserved blocks. We have probed the secondary structure of U3 snRNA in intactXenopuslaevisnuclei using single-strand specific chemical reagents; primer extension was used to map the positions of chemical modification. The three blocks of conserved sequences fall within single-stranded regions, and are therefore accessible for interaction with other molecules. Models of U3 snRNA function are discussed in light of these data.