Regulators of nitric oxide signaling triggered by host perception in a plant pathogen
Regulators of nitric oxide signaling triggered by host perception in a plant pathogen
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DOI：
10.1073/pnas.1918977117
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发表时间：
2020-05-19
影响因子：
11.1
通讯作者：
Kazan, Kemal
中科院分区：
文献类型：
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作者：
Ding, Yi;Gardiner, Donald M.;Kazan, Kemal
植物根部或病原微生物之间的根际相互作用是通过信号的相互交换启动的。然而，土壤病原体如何感知宿主信号尚不清楚。在这里，我们研究了与禾谷镰刀菌宿主识别相关的早期分子事件，禾谷镰刀菌是一种经济上重要的真菌病原体，可以感染谷类作物的根部和头部。我们发现，在与植物根部进行物理接触之前，宿主的感知会从根本上改变转录组，并触发禾谷镰刀菌中一氧化氮（NO）的产生。我们鉴定了禾谷镰刀菌中宿主介导的 NO 产生和毒力所需的含有锚蛋白重复结构域的蛋白 (FgANK1)。在没有宿主植物的情况下，FgANK1 存在于细胞质中。为了响应宿主信号，FgANK1 易位到细胞核并与锌指转录因子 (FgZC1) 相互作用，这也是禾谷镰刀菌与硝酸还原酶 (NR) 启动子特异性结合、NO 产生和毒力所必需的。我们的结果揭示了土壤病原体采用的宿主识别策略的机制见解。
The rhizosphere interaction between plant roots or pathogenic microbes is initiated by mutual exchange of signals. However, how soil pathogens sense host signals is largely unknown. Here, we studied early molecular events associated with host recognition in Fusarium graminearum, an economically important fungal pathogen that can infect both roots and heads of cereal crops. We found that host sensing prior to physical contact with plant roots radically alters the transcriptome and triggers nitric oxide (NO) production in F. graminearum. We identified an ankyrin-repeat domain containing protein (FgANK1) required for host-mediated NO production and virulence in F. graminearum. In the absence of host plant, FgANK1 resides in the cytoplasm. In response to host signals, FgANK1 translocates to the nucleus and interacts with a zinc finger transcription factor (FgZC1), also required for specific binding to the nitrate reductase(NR) promoter, NO production, and virulence in F.graminearum. Our results reveal mechanistic insights into host-recognition strategies employed by soil pathogens.