Maternal Overnutrition During Gestation in Sheep Alters Autophagy Associated Pathways in Offspring Heart.
Maternal Overnutrition During Gestation in Sheep Alters Autophagy Associated Pathways in Offspring Heart.
复制标题
DOI：
10.3389/fgene.2021.742704
复制
发表时间：
2021
影响因子：
3.7
通讯作者：
Guo W
中科院分区：
文献类型：
--
作者：
Liu Y;Ding Q;Halderson SJ;Arriola Apelo SI;Jones AK;Pillai SM;Hoffman ML;Reed S;Govoni KE;Zinn SA;Guo W
妊娠期间母亲营养不良会对后代的生长、发育以及产前和产后的健康产生负面影响。妊娠期间过度喂养或母亲肥胖（MO）会导致动物和人类的新陈代谢改变和内分泌激素失衡，这将对后代的生长和健康产生长期的有害影响。在这项研究中，我们在两个妊娠时间点和一个产后早期时间点检查了妊娠期间营养过剩对子代心肌自噬相关通路的影响（每个时间点，治疗和未治疗的男性和女性心脏分别为 n = 5）。使用双向方差分析来分析每个时间点治疗与性别之间的相互作用。我们的结果揭示了母体饮食在不同发育阶段对后代自噬信号的显着相互作用。过度喂养并不影响雄性和雌性后代在妊娠中期 90 天 (GD90) 的自噬信号传导，而 GD90 MO 雄性后代的炎症细胞因子增加；然而，妊娠期间的过度喂养显着增加了雄性和雌性的自噬信号，但在后期发育阶段（GD135和出生后第一天）的炎症水平却没有增加。我们还发现了一种性二态性反应，无论发育阶段如何，雌性后代比雄性后代更容易受到母体饮食的影响。我们还测定了男性和女性心脏在三个发育阶段的皮质醇浓度。我们没有观察到男性和女性之间或过度喂养组和对照组之间皮质醇的变化。我们的探索性研究表明，MO 会改变男性和女性在发育后期的自噬相关途径，并对女性产生更深远的影响。这一发现需要未来通过更大的样本数量来证实。我们的结果表明，针对自噬途径可能是纠正过度喂养母羊后代不利影响的策略。
Poor maternal nutrition during gestation can negatively affect offspring growth, development, and health pre- and post-natally. Overfeeding during gestation or maternal obesity (MO) results in altered metabolism and imbalanced endocrine hormones in animals and humans which will have long-lasting and detrimental effects on offspring growth and health. In this study, we examined the effects of overnutrition during gestation on autophagy associated pathways in offspring heart muscles at two gestational and one early postnatal time point (n = 5 for treated and untreated male and female heart respectively at each time point). Two-way ANOVA was used to analyze the interaction between treatment and sex at each time point. Our results revealed significant interactions of maternal diet by developmental stages for offspring autophagy signaling. Overfeeding did not affect the autophagy signaling at mid-gestation day 90 (GD90) in both male and female offspring while the inflammatory cytokines were increased in GD90 MO male offsrping; however, overfeeding during gestation significantly increased autophagy signaling, but not inflammation level at a later developmental stage (GD135 and day 1 after birth) in both males and females. We also identified a sexual dimorphic response in which female progeny were more profoundly influenced by maternal diet than male progeny regardless of developmental stages. We also determined the cortisol concentrations in male and female hearts at three developmental stages. We did not observe cortisol changes between males and females or between overfeeding and control groups. Our exploratory studies imply that MO alters autophagy associated pathways in both male and female at later developmental stages with more profound effects in female. This finding need be confirmed with larger sample numbers in the future. Our results suggest that targeting on autophagy pathway could be a strategy for correction of adverse effects in offspring of over-fed ewes.
登录
查看更多内容
影响因子：
120.7
作者：
Flegal, Katherine M.;Carroll, Margaret D.;Curtin, Lester R.
通讯作者：
Curtin, Lester R.
影响因子：
3.7
作者：
Ghnenis AB;Odhiambo JF;McCormick RJ;Nathanielsz PW;Ford SP
通讯作者：
Ford SP
影响因子：
8
作者：
Boney, CM;Verma, A;Vohr, BR
通讯作者：
Vohr, BR
影响因子：
9
作者：
通讯作者：
--
影响因子：
4.8
作者：
Djavaheri-Mergny, Mojgan;Amelotti, Manuela;Codogno, Patrice
通讯作者：
Codogno, Patrice