Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
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含葡萄糖的高甘露糖糖蛋白核心寡糖的分离。
DOI：
10.1073/pnas.81.20.6340
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发表时间：
1984
影响因子：
11.1
通讯作者：
Ballou,CE
中科院分区：
文献类型：
--
作者：
Tsai,PK;Ballou,L;Esmon,B;Schekman,R;Ballou,CE
总细胞壁甘露糖蛋白已经从酿酒酵母的突变体中分离出来，该突变体在寡糖转移到蛋白质中的天冬酰胺单元后不能去除长醇连接的前体的葡萄糖单元。通过内切氨基葡萄糖苷酶H消化从该甘露糖蛋白中释放的寡糖显示出1H NMR信号，其可归属于三个α-连接的葡萄糖单元，如δ 5.52、5.27和5.17，并且与参比化合物的化学位移比较显示这些信号与结构α Glc-2 α Glc-3 α Man-2一致。这提供了对先前分配给脂质连接的前体的结构的直接确认。对较大寡糖的分析证实，葡萄糖单元的存在不会阻止α 1- 6-连接的聚甘露糖主链的延伸或α 1- 3-连接的甘露糖添加到核心。
The total cell wall mannoprotein has been isolated from a mutant of Saccharomyces cerevisiae that fails to remove the glucose units of the dolichol-linked precursor after transfer of the oligosaccharide to asparagine units in the protein. The oligosaccharides released from this mannoprotein by endoglucosaminidase H digestion show 1H NMR signals assignable to three alpha-linked glucose units as delta 5.52, 5.27, and 5.17, and a comparison with the chemical shifts of reference compounds shows that these signals are consistent with the structure alpha Glc----2 alpha Glc----3 alpha Man----2. This provides a direct confirmation for the structure previously assigned to the lipid-linked precursor. Analysis of the larger oligosaccharides confirms that the presence of the glucose units does not prevent elongation of the alpha 1----6-linked polymannose backbone or addition of alpha 1----3-linked mannose to the core.