Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells
Sp1-dependent regulation of Myeloid Elf-1 like factor in human epithelial cells
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DOI：
10.1016/j.febslet.2005.04.015
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发表时间：
2005-05-23
期刊：
影响因子：
3.5
通讯作者：
Kai, H
中科院分区：
文献类型：
--
作者：
Koga, T;Suico, MA;Kai, H
髓样Elf-1样因子（MEF）是一种ETS蛋白，其激活粒细胞巨噬细胞集落刺激因子、白细胞介素-3、溶菌酶、人β防御素-2和穿孔素的启动子。尽管它的许多已知的功能，很少有人知道MEF的转录调控。我们克隆了人MEF基因的5 ′侧翼区，并在-204/-54处鉴定了一个TATA较少的启动子区，该启动子区含有4个推测的Sp1结合位点，其中两个是上调MEF活性所必需的。这些通过EMSA和使用RNAi或光神霉素A处理HEK 293细胞来阻断Sp1来证明。我们的研究结果表明，Sp1组成型调节MEF基因。(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Myeloid Elf-1 like factor (MEF) is an ETS protein, which activates the promoters of granulocyte macrophage colony-stimulating factor, interleukin-3, lysozyme, human beta defensin-2 and perforin. In spite of its many known functions, little is known about MEF transcriptional regulation. Here, we cloned the 5'-flanking region of human MEF gene and identified a TATA-less promoter region at -204/-54 which contains 4 putative binding sites for Sp1, two of which are essential in upregulating MEF activity. These were proven by EMSA and blocking Sp1 using RNAi or mithramycin A treatment of HEK293 cells. Our results suggest that Sp1 constitutively regulates the MEF gene. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.