Cloning and characterization of a clip domain serine protease and its homolog (masquerade) from Eriocheir sinensis
Cloning and characterization of a clip domain serine protease and its homolog (masquerade) from Eriocheir sinensis
复制标题
中华绒螯蟹剪辑域丝氨酸蛋白酶及其同源物（伪装）的克隆和表征。
DOI：
10.1016/j.fsi.2013.07.025
复制
发表时间：
2013-10-01
影响因子：
4.7
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Huang, Ying;Li, Yu-Ran;Wang, Wen
丝氨酸蛋白酶 (SP) 或 SP 同系物 (SPH) 包括夹结构域 SP (cSP) 或 SPH (cSPH) 在消化、胚胎发育、血淋巴凝固和黑化中发挥关键作用。在这项研究中，从中华绒螯蟹的肝胰腺中鉴定出一种与果蝇化妆舞会（EsMas）相似的cSP（EscSP）和一种SPH。它们都在 N 端具有剪切域，EscSP 只有 1 个剪切域，但 EsMas 有 7 个剪切域。 EscSP 和 EsMas 的 C 端分别有一个 SP 或 SP 样结构域。与 EscSP 相比，Set 催化残基的缺失导致 EsMas 的 SP 活性丧失。组织分布分析显示EscSP mRNA主要表达于肝胰腺、神经和眼柄组织；而EsMas转录本主要分布在眼柄、肌肉、神经和血细胞中。脂多糖 (LPS) 或肽聚糖 (PGN) 攻击后，血细胞中的 EscSP 显示显着增加。然而，在 LPS 攻击 2 至 24 小时的血细胞中观察到 EsMas 下调，相比之下，PGN 攻击在 2 和 24 小时可诱导 EsMas。所有这些结果表明EscSP和EsMas可能参与了中华棘球虫的先天免疫防御。 (C) 2013 Elsevier Ltd. 保留所有权利。
Serine proteinases (SPs) or SP homologs (SPHs) including clip domain SPs (cSPs) or SPHs (cSPHs) play critical roles in digestion, embryonic development, hemolymph coagulation, and melanization. In this study, one cSP (EscSP) and one SPH, similar to Drosophila masquerade (EsMas), were identified from hepatopancreas of the Chinese mittern crab Eriocheir sinensis. They both possess the clip domains at the N-terminal, EscSP has only one clip domain, but EsMas has seven clip domains. One SP or SP-like domain was at the C-terminal of EscSP and EsMas respectively. In contrast to EscSP, absence of a catalytic residue of Set resulted in the loss of SP activity of EsMas. Tissue distribution analysis showed that EscSP mRNA was mainly expressed in hepatopancreas, nerve and eyestalk tissue; whereas the EsMas transcript was mainly distributed in eyestalk, muscle, nerve and hemocytes. EscSP in hemocytes showed significant increase after a lipopolysaccharide (LPS) or peptidoglycan (PGN) challenge. However, down-regulation of EsMas was observed in hemocytes challenged by LPS from 2 to 24 h, by contrast EsMas could be induced by PGN challenge at 2 and 24 h. All these findings indicated that EscSP and EsMas might be involved in the innate immune defenses in E. sinensis. (C) 2013 Elsevier Ltd. All rights reserved.