Glutamatergic stimulation of the medial amygdala induces steroid dependent c-fos expression within forebrain nuclei responsive to mating stimulation.
Glutamatergic stimulation of the medial amygdala induces steroid dependent c-fos expression within forebrain nuclei responsive to mating stimulation.
复制标题
内侧杏仁核的谷氨酸刺激诱导对交配刺激有反应的前脑核内类固醇依赖性 c-fos 表达。
DOI：
10.1016/j.neuroscience.2005.02.053
复制
发表时间：
2005
期刊：
影响因子：
3.3
通讯作者：
Erskine,MS
中科院分区：
文献类型：
--
作者：
Lehmann,ML;Erskine,MS
已知雌性大鼠背内侧杏仁核后核内的神经元通过N-甲基-d-天冬氨酸通道激活处理交配期间收到的阴道神经刺激，将信息传递给调节神经内分泌功能的下游下丘脑细胞群。用谷氨酸、天冬氨酸和甘氨酸的兴奋性氨基酸混合物刺激这些神经元，启动10-12天的催乳素激增分泌，这通常仅在接受阴道宫颈刺激后观察到。后背内侧杏仁核神经元也含有雌激素和孕激素受体。本实验检查了参与催乳素分泌的哪些下游位点在背内侧杏仁核内谷氨酸受体激活后显示c-fos表达，以及卵巢类固醇是否影响这些区域的细胞激活。卵巢切除的雌性大鼠植入单侧插管，直接在背内侧杏仁核后接受注射的苯甲酸雌二醇和孕酮或油，然后用兴奋性氨基酸或对照PBS输注治疗。另一组苯甲酸雌二醇+炔雌醇给药的雌性动物输注1.0 μM甘氨酸单独的PBS溶液。输注给药3次，间隔30分钟。在输注后90 min，化学染色法测定输注同侧和对侧的FOS诱导。兴奋性氨基酸和激素处理可诱导3种c-fos表达模式：（1）兴奋性氨基酸和激素处理的反应区[杏仁核背内侧后核、视前内侧区、下丘脑腹外侧腹内侧核、终纹床核]，（2）苯甲酸雌二醇+孕酮处理的反应区[室周前腹侧核和背内侧核]，（3）苯甲酸雌二醇+孕酮处理的反应区[室周前腹侧核和背内侧核]，（4）苯甲酸雌二醇+孕酮处理的反应区[室周前腹侧核和背内侧核]。3）仅对兴奋性氨基酸的反应[弓状核、视交叉上核和室旁核]。这些数据确定了可能的电路，其中阴道神经刺激，通过激活背内侧杏仁核谷氨酸型受体，启动和协调一系列事件内的一个更大的神经内分泌电路重要的怀孕。
Neurons within the posterodorsal medial amygdala of female rats are known to process vaginocervical stimulation received during mating through N-methyl-d-aspartate channel activation, conveying information to downstream hypothalamic cell groups that modulate neuroendocrine function. Stimulation of these neurons with an excitatory amino acid cocktail of glutamate, aspartate and glycine initiates 10–12 days of prolactin surge secretion that normally are observed only after the receipt of vaginocervical stimulation. Posterodorsal medial amygdala neurons responsive to vaginocervical stimulation also contain estrogen and progesterone receptors. The present experiment examined which downstream sites involved in prolactin secretion show c-fos expression following glutamate receptor activation within the posterodorsal medial amygdala and whether ovarian steroids influence cellular activation in these areas. Ovariectomized female rats implanted with unilateral cannulas directed at the posterodorsal medial amygdala received injections of estradiol benzoate and progesterone or oil before infusion treatment with either excitatory amino acid or control PBS. An additional group of estradiol benzoate+progesterone-treated females was infused with 1.0 μM glycine alone in PBS. Infusions were administered three times at 30 min intervals. FOS induction 90 min after infusion was determined immunohistochemically on the sides ipsilateral and contralateral to the infusion. Of the examined regions, excitatory amino acid treatment and hormone treatment induced three patterns of c-fos expression: 1) responses to both excitatory amino acid and hormone treatment [posterodorsal medial amygdala, medial preoptic area, ventrolateral ventromedial hypothalamic nucleus, bed nucleus of the stria terminalis]; 2) responses to estradiol benzoate+progesterone treatment only [anteroventral periventricular nucleus and dorsomedial nucleus]; and 3) responses to excitatory amino acid only [arcuate nucleus, suprachiasmatic nucleus, and paraventricular nucleus]. These data identify possible circuits by which vaginocervical stimulation, via activation of posterodorsal medial amygdala glutamate-type receptors, initiates and coordinates a series of events within a larger neuroendocrine circuit important for pregnancy.