New Visible-Light Photoinitiating System for Improved Print Fidelity in Gelatin-Based Bioinks
New Visible-Light Photoinitiating System for Improved Print Fidelity in Gelatin-Based Bioinks
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DOI：
10.1021/acsbiomaterials.6b00149
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发表时间：
2016-10-01
影响因子：
5.8
通讯作者：
Woodfield, Tim B. F.
中科院分区：
文献类型：
--
作者：
Lim, Khoon S.;Schon, Benjamin S.;Woodfield, Tim B. F.
氧抑制是一种直接影响 3D 生物制造和光聚合水凝胶结构打印保真度的现象。由于交联受损，它通常会导致装配式结构发生不良的物理崩溃，并且是文献中通常未报告的问题。在这项研究中，我们描述了一种系统方法，通过比较新的可见光引发系统 Vis + 钌 (Ru)/过硫酸钠 (SPS) 与更传统采用的紫外线 (UV) + lrgacure 2959 系统，最大限度地减少光聚合明胶甲基丙烯酰 (Gel-MA) 水凝胶结构中的氧抑制。对于这两个系统，增加光引发剂浓度和光照射强度成功地减少了氧抑制。然而，UV + 12959 系统在高 12959 浓度和紫外光照射强度下都对细胞有害。 Vis + Ru/SPS 系统具有更好的细胞相容性，即使在高 Ru/SPS 浓度和可见光照射强度下长达 21 天，封装的细胞仍保持 >85% 的存活率，进一步凸显了该系统生物制造具有高形状保真度、细胞活力和代谢活性的充满细胞的构建体的潜力。
Oxygen inhibition is a phenomenon that directly impacts the print fidelity of 3D biofabricated and photopolymerized hydrogel constructs. It typically results in the undesirable physical collapse of fabricated constructs due to impaired cross-linking, and is an issue that generally remains unreported in the literature. In this study, we describe a systematic approach to minimizing oxygen inhibition in photopolymerized gelatin-methacryloyl (Gel-MA)-based hydrogel constructs, by comparing a new visible-light initiating system, Vis + ruthenium (Ru)/sodium persulfate (SPS) to more conventionally adopted ultraviolet (UV) + lrgacure 2959 system. For both systems, increasing photoinitiator concentration and light irradiation intensity successfully reduced oxygen inhibition. However, the UV + 12959 system was detrimental to cells at both high 12959 concentrations and UV light irradiation intensities. The Vis + Ru/SPS system yielded better cell cytocompatibility, where encapsulated cells remained >85% viable even at high Ru/SPS concentrations and visible-light irradiation intensities for up to 21 days, further highlighting the potential of this system to biofabricate cell-laden constructs with high shape fidelity, cell viability, and metabolic activity.