Analysis of truncated variants of the iron dependent transcriptional regulators from Corynebacterium diphtheriae and Mycobacterium tuberculosis.-猫眼课题宝

白喉棒状杆菌和结核分枝杆菌铁依赖性转录调节因子的截短变体分析。

DOI：

10.1016/j.femsle.2004.11.031

发表时间：

2005

期刊：

FEMS microbiology letters.

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

Oram,DianaMarra;Must,LisaM;Spinler,JenniferK;Twiddy,EddaM;Holmes,RandallK

白喉毒素阻遏物家族的铁依赖性调节蛋白调节多种细菌物种中的转录。这些监管机构有三个领域。结构域1和2是DNA和金属结合所必需的，而第三结构域的作用仅部分确定。我们比较了全长和羧基末端截短的变体白喉棒状杆菌DtxR和结核分枝杆菌IdeR的抗体识别，DNA结合和阻遏活性。DtxR的第三个结构域含有免疫显性表位，并且是大肠杆菌报告系统中完全阻遏物活性所必需的，但它不是体外结合DNA所必需的。相反，IdeR的第三个结构域是体外完全DNA结合活性和体内阻遏物活性所必需的。DtxR和IdeR在它们对DNA结合和阻遏物活性的结构域3的需求方面显著不同。

Iron dependent regulatory proteins of the diphtheria toxin repressor family regulate transcription in a variety of bacterial species. These regulators have three domains. Domains 1 and 2 are required for DNA- and metal-binding while the role of the third domain is only partially defined. We compared full-length and carboxyl-terminally truncated variants ofCorynebacterium diphtheriaeDtxR andMycobacterium tuberculosisIdeR for recognition by antibodies, DNA binding, and repressor activity. The third domain of DtxR contains immunodominant epitopes and is required for full repressor activity in anEscherichia colireporter system, but it is not required for binding to DNA in vitro. In contrast, the third domain of IdeR is required both for full DNA binding activity in vitro and for repressor activity in vivo. DtxR and IdeR differ significantly in their requirements for domain 3 for DNA-binding and repressor activity.