Grape seed polyphenols protect cardiac cells from apoptosis via induction of endogenous antioxidant enzymes
Grape seed polyphenols protect cardiac cells from apoptosis via induction of endogenous antioxidant enzymes
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DOI：
10.1021/jf063071b
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发表时间：
2007-03-07
影响因子：
6.1
通讯作者：
Lou, Hongxiang
中科院分区：
文献类型：
--
作者：
Du, Yu;Guo, Huaifang;Lou, Hongxiang
据报道，葡萄籽提取物（GSE）对各种形式的心脏疾病具有保护作用。GSE的心血管保护作用被认为归因于其抗氧化特性。一系列的研究表明，多酚类物质是GSE的抗氧化特性的工具。本研究旨在探讨从GSE中分离的两种主要多酚（儿茶素和原花青素B-4）是否可以增加心肌细胞中的内源性抗氧化酶，以及这种增加的细胞防御是否可以提供对氧化性心肌细胞损伤的保护。用微摩尔浓度的儿茶素或原花色素B-4孵育心脏H9 C2细胞导致细胞抗氧化酶以浓度依赖性方式显著诱导。此外，儿茶素或原花青素B-4预处理导致黄嘌呤氧化酶（XO）/黄嘌呤诱导的细胞内活性氧（ROS）积累和心肌细胞凋亡显着减少。结果表明，葡萄籽多酚（GSP）可通过诱导内源性抗氧化酶的活性来保护心肌细胞凋亡。这可能是GSE对各种形式的心血管疾病的保护作用的重要机制。
Grape seed extract (GSE) has been reported to exert protective effects on various forms of cardiac disorders. The cardiovascular protective effects of GSE are believed to be ascribed to its antioxidative properties. A series of studies have demonstrated that polyphenols are instrumental for the antioxidative properties of GSE. This study was undertaken to investigate whether two major polyphenols isolated from GSE (catechin and proanthocyanidin B-4) could increase the endogenous antioxidant enzymes in cardiomyocytes, and whether such increased cellular defenses could provide protection against oxidative cardiac cell injury. Incubation of cardiac H9C2 cells with micromolar concentrations of catechin or proanthocyanidin B-4 resulted in a significant induction of cellular antioxidant enzymes in a concentration-dependent fashion. Furthermore, catechine or proanthocyanidin B-4 pretreatment led to a marked reduction in xanthine oxidase(XO)/xanthine-induced intracellular reactive oxygen species (ROS) accumulation and cardiac cell apoptosis. These results indicated that grape seed polyphenols (GSP) could protect against cardiac cell apoptosis via the induction of endogenous antioxidant enzymes. This may be an important mechanism underlying the protective effects of GSE observed with various forms of cardiovascular disorders.