High quality RNA retrieved from samples obtained by using LMPC (laser microdissection and pressure catapulting) technology
High quality RNA retrieved from samples obtained by using LMPC (laser microdissection and pressure catapulting) technology
复制标题
DOI：
10.1078/0344-0338-00442
复制
发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Sauer, U
中科院分区：
文献类型：
--
作者：
Burgemeister, R;Gangnus, R;Sauer, U
高质量分离完整 RNA 是进行许多基础分子生物学实验的第一步，通常也是最关键的一步，对于基因表达分析中使用的许多技术至关重要。由于影响核酸保存的因素有很多，RNA 分离应在实际 RNA 提取之前和之后包括一些重要步骤。我们测试了固定和染色方案对 RNA 完整性和浓度的影响。一个经常被低估的因素是同质起始材料的绝对必要性。 LMPC 技术的应用可以快速、高精度地采购适合各种下游分析的纯化细胞群。
Isolation of intact RNA in high quality is the first and often the most critical step in performing many fundamental molecular biology experiments, and is essential for many techniques used in gene expression analysis. As many factors influence nucleic acid preservation, RNA isolation should include some important steps before and after the actual RNA extraction. We tested the influence of fixation and staining protocols regarding RNA integrity and concentration.A factor that is often underestimated is the absolute necessity for homogenous starting materials. Application of the LMPC technology allows for a rapid and highly precise procurement of purified cell populations suitable for a variety of downstream analyses.