Effect of caspases and RANKL induced by heavy force in orthodontic root resorption
Effect of caspases and RANKL induced by heavy force in orthodontic root resorption
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DOI：
10.4041/kjod.2018.48.4.253
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发表时间：
2018-07-01
影响因子：
1.9
通讯作者：
Kasai, Kazutaka
中科院分区：
文献类型：
--
作者：
Minato, Yukari;Yamaguchi, Masaru;Kasai, Kazutaka
目的：正畸牙齿移动引起的正畸牙根吸收是一种治疗困难的不良事件。半胱天冬酶是细胞凋亡的重要效应分子。目前，对牙骨质中ORR和细胞凋亡的潜在机制知之甚少。本体内研究的目的是调查的表达抗酒石酸酸性磷酸酶（TRAP），半胱天冬酶3，半胱天冬酶8，和核因子κ-B配体（RANKL）的受体激活剂在牙骨质中响应于一个沉重的或最佳的正畸力。方法：采用闭合螺旋弹簧对雄性Wistar大鼠上颌磨牙施加10 g和50 g的正畸力。在每个实验阶段的第1、3、5和7天，在施加正畸力后处死大鼠。并进行组织病理学和免疫组化分析。结果如下：在第7天，对于50 g组，苏木精和伊红染色显示许多牙根吸收陷窝，牙根上有破牙细胞，而免疫组化显示TRAP和RANKL阳性细胞增加。第3天和第5天，50 g组牙骨质表面的Caspase 3和caspase 8阳性细胞增加。此外，caspase 3-和caspase 8-阳性细胞和RANKL-阳性细胞的数量在50-g组中显著高于10-g组。结论：在我们的大鼠模型中，ORR发生在重度正畸力诱导牙骨质细胞凋亡之后。这些结果表明，成牙骨质细胞凋亡参与ORR。
Objective: Orthodontic root resorption (ORR) due to orthodontic tooth movement is a difficult treatment-related adverse event. Caspases are important effector molecules for apoptosis. At present, little is known about the mechanisms underlying ORR and apoptosis in the cementum. The aim of the present in vivo study was to investigate the expression of tartrate-resistant acid phosphatase (TRAP), caspase 3, caspase 8, and receptor activator of nuclear factor kappa-B ligand (RANKL) in the cementum in response to a heavy or an optimum orthodontic force. Methods: The maxillary molars of male Wistar rats were subjected to an orthodontic force of 10 g or 50 g using a closed coil spring. The rats were sacrificed each experimental period on days 1, 3, 5, and 7 after orthodontic force application. And the rats were subjected to histopathological and immunohistochemical analyses. Results: On day 7 for the 50-g group, hematoxylin and eosin staining revealed numerous root resorption lacunae with odontoclasts on the root, while immunohistochemistry showed increased TRAP- and RANKL-positive cells. Caspase 3- and caspase 8-positive cells were increased on the cementum surfaces in the 50-g group on days 3 and 5. Moreover, the number of caspase 3- and caspase 8-positive cells and RANKL-positive cells was significantly higher in the 50-g group than in the 10-g group. Conclusions: In our rat model, ORR occurred after apoptosis was induced in the cementum by a heavy orthodontic force. These findings suggest that apoptosis of cementoblasts is involved in ORR.