Accumulation of swimming bacteria near a solid surface
Accumulation of swimming bacteria near a solid surface
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DOI：
10.1103/physreve.84.041932
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发表时间：
2011-10-28
影响因子：
2.4
通讯作者：
Brun, Yves V.
中科院分区：
文献类型：
--
作者：
Li, Guanglai;Bensson, James;Brun, Yves V.
我们使用基于暗场显微镜的三维跟踪技术测量了向前游动的新月形茎杆菌菌株在表面附近的分布，发现游动细菌在距离表面1微米的范围内大量聚集。我们将这种积累归因于游动细胞与表面的频繁碰撞，导致它们在不断向前移动时与表面平行排列。稳定状态下的积累程度是通过平衡这些碰撞与微米大小细菌的旋转布朗运动所引起的排列来计算的。我们在此模型的基础上进行了模拟，再现了测量结果。另外的模拟表明，积累依赖于游泳速度和细胞大小，表明长而快的细胞比短而慢的细胞在表面附近积聚更多。
We measured the distribution of a forward swimming strain of Caulobacter crescentus near a surface using a three-dimensional tracking technique based on dark field microscopy and found that the swimming bacteria accumulate heavily within a micrometer from the surface. We attribute this accumulation to frequent collisions of the swimming cells with the surface, causing them to align parallel to the surface as they continually move forward. The extent of accumulation at the steady state is accounted for by balancing alignment caused by these collisions with the rotational Brownian motion of the micrometer-sized bacteria. We performed a simulation based on this model, which reproduced the measured results. Additional simulations demonstrate the dependence of accumulation on swimming speed and cell size, showing that longer and faster cells accumulate more near a surface than shorter and slower ones do.