Detection of Genetic Elements Carrying vanA in Vancomycin-Resistant Enterococcus saigonensis VE80T Isolated from Retail Chicken Meat
Detection of Genetic Elements Carrying vanA in Vancomycin-Resistant Enterococcus saigonensis VE80T Isolated from Retail Chicken Meat
复制标题
零售鸡肉中分离的耐万古霉素西贡肠球菌 VE80T 携带 vanA 遗传元件的检测
DOI：
10.1089/fpd.2020.2827
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发表时间：
2020
影响因子：
2.8
通讯作者：
and Kentaro Kawatsu
中科院分区：
文献类型：
--
作者：
Tetsuya Harada;Daisuke Motooka;Shota Nakamura;Yoshimasa Yamamoto;Takahiro Yamaguchi;Ryuji Kawahara;and Kentaro Kawatsu
在这项研究中，我们的目的是检测遗传元件携带的西贡肠球菌VE 80 T分离，从零售鸡在越南。通过vanA基因簇测序、Southern杂交分析和全基因组序列分析，检测万古霉素耐药决定簇的结构和万古霉素耐药基因的定位。与原型Tn 1546相比，Tn 1546相关元件具有5个点突变的特征性结构，其含有vanA簇，定位于76-kb质粒pVE 80 -1的VE 80 T上。VE 80 Tharboring三个点突变的VAN S序列与从台湾和日本的家禽中分离的万古霉素耐药肠球菌的序列100%相同，表明该元件可能在亚洲的家禽生产农场中流行。
In this study, we aimed to detect genetic elements carryingvanAinEnterococcus saigonensisVE80Tisolated from retail chicken in Vietnam. The structures of vancomycin-resistance determinants and the location of vancomycin-resistance genes were detected by sequencing thevanAgene cluster, Southern hybridization analyses, and whole-genome sequence analyses. The Tn1546-related elements harboringvanAclusters, which exhibited a characteristic structure with five point mutations compared with the prototype Tn1546, were located on the 76-kb plasmid pVE80-1 of VE80T. ThevanSsequence of VE80Tharboring three point mutations was 100% identical to those of vancomycin-resistant enterococci isolated from poultry in Taiwan and Japan, indicating that the element may be prevalent in poultry production farms in Asia.