A draft network of ligand-receptor-mediated multicellular signalling in human.
A draft network of ligand-receptor-mediated multicellular signalling in human.
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DOI：
10.1038/ncomms8866
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发表时间：
2015-07-22
影响因子：
16.6
通讯作者：
Forrest AR
中科院分区：
文献类型：
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作者：
Ramilowski JA;Goldberg T;Harshbarger J;Kloppmann E;Lizio M;Satagopam VP;Itoh M;Kawaji H;Carninci P;Rost B;Forrest AR
跨多种细胞类型和组织的细胞间通讯严格控制后生动物的正常功能，并广泛依赖于分泌的配体和细胞表面受体之间的相互作用。在此，我们展示了144种人类原代细胞类型之间的细胞间通讯的第一个大规模地图。我们发现，大多数细胞表达数十到数百个配体和受体，通过多个配体-受体路径创建高度连接的信号网络。我们还观察到广泛的自分泌信号，大约三分之二的伴侣可能在相同的细胞类型上相互作用。我们发现质膜和分泌蛋白具有最高的细胞类型特异性，它们比细胞内蛋白进化更年轻，并且大多数受体在其配体之前已经进化。我们提供了一个在线工具来交互式查询和可视化我们的网络，并展示了这个工具如何揭示新的细胞与细胞的相互作用，预测肥大细胞通过CSF 1-CSF 1 R相互作用对向单核细胞谱系发出信号。 细胞与细胞之间的通讯依赖于分泌的配体和细胞表面受体之间的相互作用。在这里，Ramilowski等人提出了一个基于144种不同人类细胞类型中配体-受体对表达的细胞间通讯网络草案。
Cell-to-cell communication across multiple cell types and tissues strictly governs proper functioning of metazoans and extensively relies on interactions between secreted ligands and cell-surface receptors. Herein, we present the first large-scale map of cell-to-cell communication between 144 human primary cell types. We reveal that most cells express tens to hundreds of ligands and receptors to create a highly connected signalling network through multiple ligand–receptor paths. We also observe extensive autocrine signalling with approximately two-thirds of partners possibly interacting on the same cell type. We find that plasma membrane and secreted proteins have the highest cell-type specificity, they are evolutionarily younger than intracellular proteins, and that most receptors had evolved before their ligands. We provide an online tool to interactively query and visualize our networks and demonstrate how this tool can reveal novel cell-to-cell interactions with the prediction that mast cells signal to monoblastic lineages via the CSF1–CSF1R interacting pair. Cell-to-cell communication relies upon interactions between secreted ligands and cell surface receptors. Here, Ramilowski et al. present a draft cell-to-cell communication network based on expression of ligand-receptor pairs in 144 different human cell types.