Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV
Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV
复制标题
鸡大染色体含有内源性原病毒，微染色体含有与ASV转化基因相关的序列
DOI：
10.1016/0092-8674(77)90098-8
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发表时间：
1977
期刊：
影响因子：
64.5
通讯作者：
H. Varmus
中科院分区：
文献类型：
--
作者：
Thomas Padgett;E. Stubblefield;H. Varmus
从鸡的整倍体马立克淋巴瘤细胞系中提取染色体，根据大小在区域转子中进行速率区域离心分离。利用从梯度部分提取的未标记DNA和体外制备的标记单链病毒特异性DNA进行的DNA-DNA杂交试验表明，大的大染色体中含有鸡内源性RNA肿瘤病毒（RAV-0）的原病毒，而与禽肉瘤病毒（ASV）转化基因相关的细胞序列位于微染色体中。为了支持该方法，我们还表明卵清蛋白的单基因可以分配到大染色体上。
Chicken chromosomes from a euploid Marek’s lymphoma cell line have been partially fractionated according to size by rate zonal centrifugation in a zonal rotor. DNA-DNA hybridization tests, using unlabeled DNA extracted from gradient fractions and labeled single-stranded, virus-specific DNAs prepared in vitro, indicate that large macrochromosomes harbor the provirus for the endogenous RNA tumor virus of chickens (RAV-0), whereas a cellular sequence related to the transforming gene of avian sarcoma virus (ASV) is located in microchromosomes. In support of the method, we have also shown that the single gene for ovalbumin can be assigned to macrochromosomes.