The generation and validation of a dual cardiac HAND1-Tomato NKX2-5-GFP human embryonic stem cell line UMANe002-A-3
The generation and validation of a dual cardiac HAND1-Tomato NKX2-5-GFP human embryonic stem cell line UMANe002-A-3
复制标题
双心脏 HAND1-Tomato NKX2-5-GFP 人胚胎干细胞系 UMANe002-A-3 的生成和验证
DOI：
10.1016/j.scr.2024.103342
复制
发表时间：
2024
影响因子：
1.2
通讯作者：
Lynch A
中科院分区：
文献类型：
--
作者：
Lynch A
转录因子HAND 1是心脏发育的关键调节因子，其在心脏祖细胞和心肌细胞的亚群中表达。相反，转录因子NKX 2 -5在心肌细胞中表达更广泛。在这里，我们报告了一个双报告人胚胎干细胞系的产生，这些基因的表达可以同时测量，使谱系分析以及研究HAND 1和NKX 2 - 5基因调控和蛋白质功能。该工具将具有广泛的实用性，特别是在发育生物学和疾病建模方面的研究。
The transcription factor HAND1 is a critical regulator of cardiac development which is expressed in sub-populations of cardiac progenitors and cardiomyocytes. The transcription factor NKX2-5, in contrast, is expressed more widely in cardiac cells. Here we report the generation of a dual reporter hESC line where the expression of these genes can be simultaneously measured, enabling lineage analysis as well as studies ofHAND1andNKX2-5gene regulation and protein function. This tool will have wide utility particularly for research on developmental biology and disease modelling.