Nanoindentation time-dependent deformation/recovery suggestive of methylglyoxal induced glycation in calcified nodules
Nanoindentation time-dependent deformation/recovery suggestive of methylglyoxal induced glycation in calcified nodules
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纳米压痕时间依赖性变形/恢复提示钙化结节中甲基乙二醛诱导的糖化
DOI：
10.1016/j.nano.2017.07.003
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发表时间：
2017
期刊：
影响因子：
5.5
通讯作者：
Kamijo Ryutaro
中科院分区：
文献类型：
--
作者：
Rodriguez Reena;Yoshimura Kentaro;Shibata Yo;Miyamoto Yoichi;Tanaka Reina;Uyama Risa;Sasa Kiyohito;Suzuki Dai;Miyazaki Takashi;Kamijo Ryutaro
虽然经验发现表明2型糖尿病患者骨折风险增加，但尚未通过物质水平获得的结果证明。在这里，我们报告的证据表明，在2型糖尿病中，在甲基乙二醛（MG）诱导的糖化方面，模拟骨转换的体外钙化结节的纳米级时间依赖性变形/恢复。纳米压痕测试结果显示，钙化结节与MG培养没有表现出足够的尺寸恢复，尽管在恒定载荷压痕测试过程中的大蠕变速率。这种较低的回收率可能是基于作为MG次级产物的晚期糖基化终产物（AGEs）形成的线性基质聚合网络。由于在2型糖尿病病例中观察到血清MG升高和与AGEs量相关的骨转换异常，因此这种时间依赖性行为可能是受影响患者材料水平上骨折机制的因素之一。
Although empirical findings have indicated increase in bone fracture risk in type 2 diabetes patients, that has yet to be proven by results obtained at the material level. Here, we report evidence showing nanoscale time-dependent deformation/recovery ofin vitrocalcified nodules mimicking bone turnover in type 2 diabetes in respect to methylglyoxal (MG)-induced glycation. Nanoindentation test results revealed that calcified nodules cultured with MG did not show adequate dimensional recovery, despite a large creep rate during constant load indentation testing. This lesser recovery is likely based on the linear matrix polymerization network formed by advanced glycation end products (AGEs) as a secondary product of MG. Since elevated serum MG and abnormal bone turnover related to the amount of AGEs are observed in cases of type 2 diabetes, this time-dependent behavior may be one of the factors of the bone fracture mechanism at the material level in affected patients.