NUCLEOTIDE-SEQUENCE OF THE REGULATORY LOCUS CONTROLLING EXPRESSION OF BACTERIAL GENES FOR BIOLUMINESCENCE
NUCLEOTIDE-SEQUENCE OF THE REGULATORY LOCUS CONTROLLING EXPRESSION OF BACTERIAL GENES FOR BIOLUMINESCENCE
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DOI：
10.1093/nar/15.24.10455
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发表时间：
1987-12-23
影响因子：
14.9
通讯作者：
SILVERMAN, M
中科院分区：
文献类型：
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作者：
ENGEBRECHT, J;SILVERMAN, M
海洋细菌费氏弧菌和含有克隆luxgenes的重组宿主的光的产生受培养物的密度控制。通量基因表达的密度依赖性调控需要一个由luxRandluxI基因和两个紧密相连的趋异启动子组成的基因座。作为了解生物发光调节的遗传分析的一部分，我们对包含这种控制电路的DNA区域进行了测序。确定了对应toluxRandluxI的开放阅读框;通过S1核酸酶图谱确定了转录起始位点，并定位了类似启动子元件的序列。
Production of light by the marine bacteriumVibrio fischeriand by recombinant hosts containing clonedluxgenes is controlled by the density of the culture. Density-dependent regulation ofluxgene expression has been shown to require a locus consisting of theluxRandluxIgenes and two closely linked divergent promoters. As part of a genetic analysis to understand the regulation of bioluminescence, we have sequenced the region of DNA containing this control circuit. Open reading frames corresponding toluxRandluxIwere identified; transcription start sites were defined by S1 nuclease mapping and sequences resembling promoter elements were located.