Inhibitory effects of luteinizing hormone releasing hormone (LH-RH) on neurons in the organum vasculosum lamina terminalis (OVLT)
Inhibitory effects of luteinizing hormone releasing hormone (LH-RH) on neurons in the organum vasculosum lamina terminalis (OVLT)
复制标题
黄体生成素释放激素 (LH-RH) 对血管器终板 (OVLT) 神经元的抑制作用
DOI：
10.1016/0006-8993(79)90390-1
复制
发表时间：
1979
期刊：
影响因子：
2.9
通讯作者：
M. Phillips
中科院分区：
文献类型：
--
作者：
D. Felix;M. Phillips
黄体生成素(LH)的释放受黄体生成素释放激素(LH-RH)的控制。该肽已通过多种解剖学技术进行了研究，到目前为止，在从小鼠到人的几个不同物种中的所有研究都一致认为，终板血管器官中含有Lh-RH免疫反应过程1、2、1、17。关于这些过程起源于哪里以及含有Lh-RH的核周是否实际上定位于OVLT 1、17，尚有争议。然而，该肽在神经过程中的一致定位表明，Lh-RH对OVLT区域的神经元具有生理作用。在这篇报道中，我们通过微离子导入的方法将这种多肽直接应用于OVLT中的神经元，研究了这种多肽对LH-RH的神经生理反应。以血管紧张素II作为对照。实验对象为成年、雌性、未去卵巢的大鼠18只。为了准确地接近终板，同时又不损伤终板，采用腹侧手术入路。4只大鼠股静脉插管采用PE50管。当硬脑膜和软脑膜从视交叉充分清除时，准备工作就准备好了。在这一过程中，通常几乎没有出血，动物在实验期间存活得很好。在整个记录和测试过程中，体温保持在36摄氏度，心率被监测。用含4M氯化钠的5管玻璃微吸管记录细胞外动作电位。尖端直径约为4#m，电阻为4-12mfl。每个神经元的放电速率由一个比率计量器(Nuclear Enterprises NE4667)计数，并连续显示在紫外线示波器(Bell and Howell 5-137)上。微管的其他通道含有微离子发射的化合物：促黄体生成素释放激素(Beckman，10 mM，pH 5.5)；血管紧张素II(CalBioChem)，在最终pH为4.5的蒸馏水中配制成10~M溶液，用NaOH调节；对多肽的反应标准是发射频率改变20~或更多。只对稳定的单元进行了测试。这些多肽被阳离子电流喷射出来。用促黄体生成素或卵泡刺激素对细胞进行检测，每隔1-2次降雨
The release of luteinizing hormone (LH) is controlled by luteinizing-hormonereleasing hormone (LH-RH), a decapeptide. This peptide has been studied by a variety of anatomical techniques and all studies so far in several different species from mouse to man are in agreement that the organum vasculosum of the lamina terminalis (OVLT) contains LH-RH immuno-reactive processes 1, 2, 1, 17. It is debatable where these processes arise from and whether perikarya containing LH-RH are actually localized in the OVLT 1, 17. Nevertheless, the consistent localization of the peptide in neural processes suggests that LH-RH has a physiological action on neurons in the OVLT regions. In this report, we have investigated neurophysiological responses to LH-RH by applying the peptide directly to neurons in the OVLT by microiontophoresis. As a control peptide we used angiotensin II. The subjects were 18 adult, female, non-ovariectomized albino rats. In order to approach the lamina terminalis accurately, but without damaging it, a ventral surgical approach was performed. In 4 rats, the femoral vein was catheterized with PE 50 tubing. When dura and pia mater has been adequately cleared from the optic chiasm, the preparation was ready for experimentation. There was usually little bleeding during this procedure and animals survived in good condition for the duration of the experiment. Temperature was maintained at 36 C and heart rate monitored throughout the recording and testing. Extracellular action potentials were recorded through the 4 M NaC1 containing barrel of a 5-barrel glass micropipette. The tip diameter was approximately 4# m with a resistance of 4-12 Mfl. The rate of firing of each neuron was counted by a ratemeter (Nuclear Enterprises NE 4667) and displayed continuously on a UV-oscillograph (Bell and Howell 5-137). The other channels of the micropipettes contained the compounds to be ejected microiontophoretically: Luteinizing hormone-releasing hormone (Beckman, 10 mM, pH 5.5); angiotensin II (Calbiochem.), prepared as a 10-~ M solution in distilled water with a final pH of 4.5, adjusted with NaOH; the criterion for a response to the peptides was a change of 20~ or more in the firing rate. Only stable units were tested. The peptides were ejected with cationic currents. Cells were tested by application of LH-RH or AII for 1-2 rain with intervals