THE SYNERGISTIC EFFECTS OF RHODAMINE-123 AND MEROCYANINE-540 LASER-DYES ON HUMAN TUMOR-CELL LINES - A NEW APPROACH TO LASER PHOTOTHERAPY
THE SYNERGISTIC EFFECTS OF RHODAMINE-123 AND MEROCYANINE-540 LASER-DYES ON HUMAN TUMOR-CELL LINES - A NEW APPROACH TO LASER PHOTOTHERAPY
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DOI：
10.1177/019459989310800305
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发表时间：
1993-03-01
影响因子：
3.4
通讯作者：
SOUDANT, J
中科院分区：
文献类型：
--
作者：
CASTRO, DJ;SAXTON, RE;SOUDANT, J
正在测试许多新的光敏剂和激光波长，以通过增强特异性肿瘤摄取和/或保留、降低全身毒性和增加激光组织穿透来改善光动力疗法。在这项研究中，罗丹明-123（Rh-123）和汞-540（MC-540）的人肿瘤细胞株激光照射后的潜在协同效应进行了探讨。在第一系列实验中，在三种人白血病细胞系（K562、RAJI、729 HF 2）、P3鳞状细胞癌和M26黑色素瘤上测试了Rh-123和M-540的摄取动力学。我们的结果表明这些细胞系对MC-540（K562 > P3 > RAJI > 729 HF 2> M26）和Rh-123（P3 > RAJI > 729 HF 2> K562 > M26）的摄取速率和量存在明显差异。在第二系列实验中，M26肿瘤细胞用Rh-123（1 μ g/ml）或MC-540（20 μ g/ml）单独或与两种染料的组合敏化60分钟，然后暴露于非热能水平的氩（514.5 nm）loser。我们的研究结果表明，与单独使用两种染料（MC-540和Rh-123）相比，激光对M26癌细胞的杀瘤作用显著增强。与联合抗生素治疗一样，具有不同细胞内靶向位点的两种激光染料的协同作用似乎在暴露于匹配的激光波长后显著增强了杀肿瘤作用。这些数据提供了证据，有效的激光光疗法的染料协同作用。
Many new photosensitizers and laser wavelengths are being tested to improve photodynamic therapy by enhancing specific tumor uptake and/or retention, lowering systemic toxicity, and increasing laser tissue penetration. In this study the potential synergistic effects of rhodamine-123 (Rh-123) and merocyanine-540 (MC-540) sensitization of human tumor cell lines after laser exposure were explored. In a first series of experiments, the kinetics of uptake of Rh-123 and M-540 were tested on three human leukemia cell lines (K562, RAJI, 729HF2), P3 squamous carcinoma, and M26 melanoma. Our results demonstrate a clear difference in the rate and amount of uptake of MC-540 (K562 > P3 > RAJI > 729HF2 > M26) and Rh-123 (P3 > RAJI > 729HF2 > K562 > M26) by these cell lines. In a second series of experiments, M26 tumor cells were sensitized with either Rh-123 (1 mug/ml) or with MC-540 (20 mug/ml) alone or with a combination of the two dyes for 60 minutes, then exposed to the argon (514.5 nm) loser at nonthermal energy levels. Our results demonstrate a significant enhancement of the tumoricidal effects of the laser on M26 carcinoma cells after sensitization with both dyes together (MC-540 and Rh-123) when compared to each dye alone. As with combination antibiotherapy, the synergistic effects of two laser dyes that have different intracellular targeting sites appear to enhance tumoricidal effects significantly after exposure to a matching laser wavelength. The data provide evidence for effective laser phtototherapy by dye synergy.