Protective effect of silicon on phenolic biosynthesis and ultraviolet spectral stress in rice crop
Protective effect of silicon on phenolic biosynthesis and ultraviolet spectral stress in rice crop
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DOI：
10.1016/s0168-9452(02)00419-3
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发表时间：
2003-03-01
期刊：
影响因子：
5.2
通讯作者：
Morita, O
中科院分区：
文献类型：
--
作者：
Goto, M;Ehara, H;Morita, O
用紫外显微光谱法和免疫显微镜法对水稻叶片细胞中肉桂醇氢化酶（CAD）进行了研究。通过X射线微区分析仪对水稻叶片纵向表面硅的分布模式、形态特征以及总组成如硅、其他主要矿物元素、纤维素、非纤维素多糖、木质素和酚酸进行了比较。X射线微区硅分析表明，与-Si水稻植株相比，+Si水稻植株的哑铃体和其他表面积上硅的存款量较高。+Si水稻植株厚壁组织、维管束鞘和后生木质部导管细胞壁对CAD抗血清的免疫反应也较低。+Si水稻植株叶片在280和320 nm附近的紫外吸收率低于-Si水稻植株。两组水稻叶片的显微观察结果也与酚酸检测结果一致。这项研究表明，硅施肥和/或硅酸盐在叶片中的积累可以与酚类生物合成的减少在水稻植株。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
A work of ultraviolet microspectrometry and immuno light microscopy of cinnamyl alcohol hydrogenase (CAD) in the cells of leaf blades was carried out on rice plants grown in a paddy field with (+Si) or without (-Si) application of a silicate fertilizer. The pattern of silicon distribution on the longitudinal surface of leaf blades by X-ray microanalyzer, morphological characteristics, and gross composition such as silica, other major mineral elements, cellulose, non-cellulosic polysaccharides, lignin and phenolic acids were also compared between both rice plants. The X-ray microanalysis of silicon indicated the higher deposit on dumbbell bodies and other surface area in the +Si rice plants compared with those of the -Si rice plants. The +Si rice plants also showed the lower immuno-reaction of antiserum of the CAD in the cell walls of sclerenchyma, vascular bundle sheath and metaxylem vessel cells. The +Si rice plants had the lower UV absorbance around 280 and 320 nm in the leaf blades compared with those of the -Si rice plants. Microscopic observations of leaf blades in both sets of rice plants were also consistent with results of phenolic acids examined. This study suggests that silica fertilization and/or silicate accumulation in the leaves can be associated with decreases of the phenolic biosynthesis in rice plants. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.