An investigation of factors influencing mitotic G2 delay in synchronous cultures of human kidney cells after x-irradiation.
An investigation of factors influencing mitotic G2 delay in synchronous cultures of human kidney cells after x-irradiation.
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X 射线照射后人肾细胞同步培养物中影响有丝分裂 G2 延迟的因素的研究。
DOI：
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发表时间：
1969
期刊：
影响因子：
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通讯作者：
H. Brohee
中科院分区：
文献类型：
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作者：
J. Scaife;H. Brohee
Factors affecting mitosis have been studied in human kidney T-cells synchronized by two exposures to 7.5 mM thymidine. Biochemical measurements were correlated with the position of the cells in the cell cycle from S to mitosis. Anaerobic conditions during S, although not inhibition of respiration, prevented cells from progressing to mitosis. ATP production is largely anaerobic and glycolytic metabolism is sufficient for a cell to complete mitosis once it has entered G2 or prophase. Both RNA and protein synthesis are required in G2 for the initiation of cell division, while treatment with carbon monoxide or thiol blocking agents also prevents mitosis.Postirradiation mitotic delay is greater for cells irradiated in G2 than for cells irradiated in S. This G2 delay is reversible if the cells are blocked in S by addition of excess thymidine. Pretreatment with 5–15 mM cysteamine largely protected irradiated cells from mitotic delay. Cysteine at a concentration of 20 mM and AET at 10 mM were only weakly protecti...
