Bactericidal effects of a high-power red light-emitting diode on two periodontopathic bacteria in antimicrobial photodynamic therapy in vitro
Bactericidal effects of a high-power red light-emitting diode on two periodontopathic bacteria in antimicrobial photodynamic therapy in vitro
复制标题
高功率红色发光二极管在体外抗菌光动力治疗中对两种牙周病细菌的杀菌作用
DOI：
10.1111/j.2041-1626.2011.00071.x
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发表时间：
2011
影响因子：
--
通讯作者：
Ishikawa I
中科院分区：
文献类型：
--
作者：
Umeda M;Tsuno A;Okagami Y;Tsuchida F;Izumi Y;Ishikawa I
目的：发光二极管已被研究为用于光动力疗法的新型光激活剂。我们研究了高功率红光发光二极管对两种牙周病细菌的体外杀菌效果。方法：使用发光二极管（强度：1100 mW/cm 2，峰值波长：650 nm）照射细菌溶液10或20 s。 细菌溶液（牙龈卟啉单胞菌或伴放线菌聚集杆菌），浓度为2.5 × 106 c.f.u./ 将mL与等体积的亚甲基蓝或甲苯胺蓝O（0-20 μg/mL）混合，并添加到滴定板威尔斯孔中。 用红色发光二极管从22或40 mm的距离照射平板威尔斯孔。 稀释内容物，并将50 μL涂抹在血琼脂平板上。 培养1周后，细菌c.f.u.结果：照射10 s和20 s后，发光二极管能量密度估计分别约为4 J/cm 2和8 J/cm 2。 结论：高功率、低浓度的红光发光二极管照射对两种牙周病致病菌均有较好的杀灭作用。
Aim:Light‐emitting diodes have been investigated as new light activators for photodynamic therapy. We investigated the bactericidal effects of high‐power, red light‐emitting diodes on two periodontopathic bacteriain vitro.Methods:A light‐emitting diode (intensity: 1100 mW/cm2, peak wavelength: 650 nm) was used to irradiate a bacterial solution for either 10 or 20 s. Bacterial solutions (Porphyromonas gingivalisorAggregatibacter actinomycetemcomitans) at a concentration of 2.5 × 106c.f.u./mL were mixed with an equal volume of either methylene blue or toluidine blue O (0–20 μg/mL) and added to titer plate wells. The plate wells were irradiated with red light‐emitting diode light from a distance of 22 or 40 mm. The contents were diluted, and 50 μL was smeared onto blood agar plates. After 1 week of culturing, bacterial c.f.u. were counted.Results:The light‐emitting diode energy density was estimated to be approximately 4 and 8 J/cm2after 10 and 20 s of irradiation, respectively. Red light‐emitting diode irradiation for 10 s from a distance of 22 mm, combined with methylene blue at concentrations >10 μg/mL, completely killedPorphyromonas gingivalisandAggregatibacter actinomycetemcomitans.Conclusion:High‐power, red light‐emitting diode irradiation with a low concentration of dye showed effective bactericidal effects against two periodontopathic bacteria.