VE-cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells
VE-cadherin-Cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells
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DOI：
10.1002/dvdy.20643
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发表时间：
2006-03-01
影响因子：
2.5
通讯作者：
Iruela-Arispe, ML
中科院分区：
文献类型：
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作者：
Alva, JA;Zovein, AC;Iruela-Arispe, ML
通过Cre/loxP系统将基因缺失靶向特定细胞区室的能力已经成为分析广泛表达基因的有力工具。在这里，我们报告了一种转基因小鼠系的产生，其中Cre重组酶的表达是在VE-钙粘蛋白启动子的调控下。时间分布和活性的酶进行了评价与两个独立的Cre报告线。在整个发育过程中和成年期进行组织学分析。早在E7.5卵黄囊内皮细胞中就检测到loxP位点的表达，随后表达β-半乳糖苷酶或GFP。从E8.5-13.5注意到胚胎脉管系统的进行性染色;然而，在所有内皮隔室（包括动脉、静脉和毛细血管）中仅在E14.5以后观察到更连续的报告基因表达。此外，我们发现淋巴管内皮细胞中的Cre活性。与其他内皮特异性Cre小鼠不同，该模型显示在成年静止血管中表达。此外，VE-钙黏着蛋白启动子在成人中的组成性质对于分析病理环境中的基因缺失可能是有利的。
The ability to target gene deletion to a specific cellular compartment via the Cre/loxP system has been a powerful tool in the analysis of broadly expressed genes. Here, we report the generation of a transgenic mouse line in which expression of Cre-recombinase is under the regulatory control of the VE-Cadherin promoter. Temporal distribution and activity of the enzyme was evaluated with two independent Cre reporter lines. Histological analysis was performed throughout development and in the adult. Recombination of lox P sites with subsequent expression of p-galactosidase or GFP was detected as early as E7.5 in endothelial cells of the yolk sac. Progressive staining of the embryonic vasculature was noted from E8.5-13.5; however, more contiguous reporter expression was only seen by E14.5 onward in all endothelial compartments including arteries, veins, and capillaries. In addition, we found Cre activity in lymphatic endothelial cells. Unlike other endothelial-specific Cre mice, this model showed expression in the adult quiescent vasculature. Furthermore, the constitutive nature of the VE-Cadherin promoter in the adult can be advantageous for analysis of gene deletion in pathological settings.