Venom gland transcriptome from Heloderma horridum horridum by high-throughput sequencing
Venom gland transcriptome from Heloderma horridum horridum by high-throughput sequencing
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DOI：
10.1016/j.toxicon.2020.04.003
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发表时间：
2020-06-01
期刊：
影响因子：
2.8
通讯作者：
Gonzalez-Carrillo, Gabino
中科院分区：
文献类型：
--
作者：
Jareth Lino-Lopez, Gisela;Leticia Valdez-Velazquez, Laura;Gonzalez-Carrillo, Gabino
Helodermatidae（Anguimorpha）家族的蜥蜴至少包括两个公认的物种：Helodermatidum horridum和Helodermatidum suspectum suspectum。它们的颚部有专门的腺体，可以产生有毒的分泌物，使被咬的动物产生中毒症状。研究来自这种分泌物的蛋白质的一种方法是通过RNA-seq;一种强大的分子工具来表征这种专门腺体的转录组及其蛋白质分泌物。从中华绒螯蟹毒腺组织中提取总RNA。提取herridum horridum的cDNA，构建cDNA文库并测序。总体而言，发现了114，172个转录本，并且基于与先前描述的肽/蛋白质的序列相似性注释了199个转录本。编码假定exendins，防御素，促钠排泄剂和丝氨酸蛋白酶抑制剂的转录本表达最高。此外，还发现了编码丝氨酸蛋白酶、磷脂酶、金属蛋白酶、脂肪酶、L-氨基氧化酶和核酸酶的转录本，其中一些新的转录本是受免疫控制的肿瘤蛋白、毒液因子、vespryns、waprins、凝集素、半胱氨酸蛋白酶抑制剂和丝氨酸蛋白酶抑制剂。所有这些新的蛋白质结构可能有助于更好地了解Helodermatidae家族的有毒分泌物。
Lizards of the Helodermatidae (Anguimorpha) family consist of at least two well recognized species: Heloderma horridum horridum and Heloderma suspectum suspectum. They contain specialized glands in their jaws that produce venomous secretions that causes envenoming symptoms to bitten animals. One way to study proteins from such secretions is by RNA-seq; a powerful molecular tool to characterize the transcriptome of such specialized gland, and its protein secretions. The total RNA from venom gland tissues of H. horridum horridum was extracted and a cDNA library was constructed and sequenced. Overall, 114,172 transcripts were found, and 199 were annotated based on sequence similarities to previously described peptides/proteins. Transcripts coding for putative exendins, defensins, natriuretics and serine protease inhibitors were the most highly expressed. Transcripts that code for several putative serine proteases, phospholipases, metalloproteases, lipases, L-amino oxidase and nucleases were also found. Some of the novel identified transcripts were translationally controlled tumor proteins, venom factors, vespryns, waprins, lectins, cystatins and serine protease inhibitors. All these new protein structures may contribute to a better understanding of the venomous secretions of the Helodermatidae family.