Preparation of amino-modified titanate nanotubes and its striking adsorption ability to duplex DNA
Preparation of amino-modified titanate nanotubes and its striking adsorption ability to duplex DNA
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DOI：
10.1007/s11051-010-0087-z
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发表时间：
2011
影响因子：
2.5
通讯作者：
Hongyun Niu;Yaqi Cai
中科院分区：
文献类型：
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作者：
Hongyun Niu;Yaqi Cai
在这项研究中，氨基修饰的钛酸盐纳米管（NH 2-TNs）的制备和用于吸附双链DNA。DNA和NH_2-TNs之间的宏观复合物被观察到。NH_2-TNs对DNA的吸收非常快;在pH 3.6-5.0下，在10 min内达到平衡。NH 2-TNs的吸附等温线中的平衡容量为240 mg g-1，通过向溶液中添加Ca 2+，吸附容量进一步增加。用磷酸盐缓冲液（PBS）溶液可以释放吸附的DNA，而不破坏DNA链。NH 2-TNs能有效地保护DNA在DNase I存在下不被切割。
In this study, amino-modified titanate nanotubes (NH2-TNs) were prepared and used to adsorb duplex DNA. Macroscopic complexes between DNA and NH2-TNs were observed. The uptake of DNA by NH2-TNs was very fast; equilibrium was reached within 10 min at pH 3.6–5.0. The equilibrium capacity in the adsorption isotherm of NH2-TNs was 240 mg g−1, and further increase of the adsorption capacity was obtained with the addition of Ca2+in solution. The adsorbed DNA could be released with phosphate buffer saline (PBS) solution without damage of the DNA strands. NH2-TNs could protect DNA from cleavage efficiently in the presence of DNase I.