A generalized fecal glucocorticoid assay for use in a diverse array of nondomestic mammalian and avian species
A generalized fecal glucocorticoid assay for use in a diverse array of nondomestic mammalian and avian species
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DOI：
10.1006/gcen.2000.7557
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发表时间：
2000-12-01
影响因子：
2.7
通讯作者：
Monfort, SL
中科院分区：
文献类型：
--
作者：
Wasser, SK;Hunt, KE;Monfort, SL
非侵入性粪便糖皮质激素分析作为评估野生动物与环境干扰相关的应激的一种手段具有巨大的潜力。然而，种间变异排泄糖皮质激素代谢产物需要仔细选择用于其定量的抗体。我们比较了四种抗体检测后的H-3皮质醇管理，促肾上腺皮质激素的挑战，和粪便糖皮质激素代谢产物的HPLC分离黄色狒狒的主要粪便皮质醇代谢产物。在放射性标记输注和ACTH激发后，最有效的抗体（ICN皮质酮RIA;目录号07-120102）对排泄峰值期间粪便中存在的主要皮质醇代谢产物表现出相对较高的交叉反应性。在非洲象、黑犀牛、罗斯福麋鹿、gerenuk、弯角大羚羊、阿拉斯加海獭、马来太阳熊、猎豹、云豹、长尾猕猴和北方斑点猫头鹰中，同样的抗体也检测到ACTH给药后粪便糖皮质激素代谢物增加。结果表明：（1）粪便糖皮质激素测定法可可靠地检测各种物种肾上腺活性的内源性变化;（2）进行比较时，ICN皮质酮抗体通常上级其他抗体，可用于测量粪便中的糖皮质激素代谢产物。(C)北京大学出版社.
Noninvasive fecal glucocorticoid analysis has tremendous potential as a means of assessing stress associated with environmental disturbance in wildlife. However, interspecific variation in excreted glucocorticoid metabolites requires careful selection of the antibody used in their quantification. We compared four antibodies for detecting the major fecal cortisol metabolites in yellow baboons following H-3 cortisol administration, ACTH challenge, and HPLC separation of fecal glucocorticoid metabolites. The most effective antibody (ICN corticosterone RIA; Cat. No. 07-120102) demonstrated relatively high cross-reactivities to the major cortisol metabolites present in feces during peak excretion, following both radiolabel infusion and ACTH challenge. This same antibody also detected increased fecal glucocorticoid metabolites after ACTH administration in the African elephant, black rhinoceros, Roosevelt elk, gerenuk, scimitar-horned oryx, Alaskan sea otter, Malayan sun bear, cheetah, clouded leopard, longtailed macaque, and northern spotted owl. Results suggest that (1) fecal glucocorticoid assays reliably detect endogenous changes in adrenal activity of a diverse array of species and (2) where comparisons were made, the ICN corticosterone antibody generally was superior to other antibodies for measuring glucocorticoid metabolites in feces. (C) 2000 Academic Press.