Tilt in Place Microscopy: a Simple, Low-Cost Solution to Image Neural Responses to Body Rotations.
Tilt in Place Microscopy: a Simple, Low-Cost Solution to Image Neural Responses to Body Rotations.
复制标题
DOI：
10.1523/jneurosci.1736-22.2022
复制
发表时间：
2023-02-08
影响因子：
5.3
通讯作者：
Schoppik, David
中科院分区：
文献类型：
--
作者：
Hamling, Kyla R.;Zhu, Yunlu;Auer, Franziska;Schoppik, David
动物利用关于重力和其他不稳定力的信息来平衡和在环境中导航。测量大脑对这些力量的反应需要大量的技术知识和/或财政资源。我们提出了一种简单的替代方法-原位显微镜（TIPM），这是一种低成本和非侵入性的方法来测量身体方向快速变化后的神经活动。在这里，我们使用TIPM研究前庭脊髓神经元在幼斑马鱼期间和之后立即滚动倾斜。前庭脊髓神经元的反应与可靠的活动增加，不同的同侧倾斜幅度的函数。TIPM分化的滋补品（即，持续倾斜），揭示了前庭外侧核中刺激敏感性的粗糙地形。相对于试验间的变异性，重复训练的神经元变异性较小，这使我们能够使用TIPM对两个发育时间点的相同神经元进行纵向研究。在那里，我们观察到反应强度的整体增加和刺激方向的神经表征的系统性变化。我们的数据扩展了经典表征的身体倾斜表示前庭脊髓神经元和建立效用的TIPM研究神经基础的平衡，特别是在发展中的动物。前庭感觉影响从导航到内感受的一切。在这里，我们详细介绍了一种简单，有效，几乎通用的方法来成像神经系统如何感知和响应身体倾斜。我们使用我们的新方法来复制和扩展过去的研究结果倾斜感应的一个保守的人口脊髓投射前庭神经元。我们方法的简单性和广泛的兼容性将使大脑对不稳定的反应的研究民主化，特别是在整个发展过程中。
Animals use information about gravity and other destabilizing forces to balance and navigate through their environment. Measuring how brains respond to these forces requires considerable technical knowledge and/or financial resources. We present a simple alternative—Tilt In Place Microscopy (TIPM), a low-cost and noninvasive way to measure neural activity following rapid changes in body orientation. Here, we used TIPM to study vestibulospinal neurons in larval zebrafish during and immediately after roll tilts. Vestibulospinal neurons responded with reliable increases in activity that varied as a function of ipsilateral tilt amplitude. TIPM differentiated tonic (i.e., sustained tilt) from phasic responses, revealing coarse topography of stimulus sensitivity in the lateral vestibular nucleus. Neuronal variability across repeated sessions was minor relative to trial-to-trial variability, allowing us to use TIPM for longitudinal studies of the same neurons across two developmental time points. There, we observed global increases in response strength and systematic changes in the neural representation of stimulus direction. Our data extend classical characterization of the body tilt representation by vestibulospinal neurons and establish the utility of TIPM to study the neural basis of balance, especially in developing animals. SIGNIFICANCE STATEMENT Vestibular sensation influences everything from navigation to interoception. Here, we detail a straightforward, validated, and nearly universal approach to image how the nervous system senses and responds to body tilts. We use our new method to replicate and expand on past findings of tilt sensing by a conserved population of spinal-projecting vestibular neurons. The simplicity and broad compatibility of our approach will democratize the study of the response of the brain to destabilization, particularly across development.
影响因子：
3.4
作者：
Straka H;Paulin MG;Hoffman LF
通讯作者：
Hoffman LF