TARGETED DISRUPTION OF VINCULIN GENES IN F9 AND EMBRYONIC STEM-CELLS CHANGES CELL MORPHOLOGY, ADHESION, AND LOCOMOTION
TARGETED DISRUPTION OF VINCULIN GENES IN F9 AND EMBRYONIC STEM-CELLS CHANGES CELL MORPHOLOGY, ADHESION, AND LOCOMOTION
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DOI：
10.1073/pnas.92.20.9161
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发表时间：
1995-09-26
影响因子：
11.1
通讯作者：
ADAMSON, ED
中科院分区：
文献类型：
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作者：
COLL, JL;BENZEEV, A;ADAMSON, ED
Vinculin是焦点粘连和zonula粘连连接的主要成分，被认为参与将微丝连接到细胞-底物和细胞-细胞接触区域，为了测试vinculin在细胞黏附和运动中的作用，我们使用同源重组产生了F9胚胎癌和突变的胚胎干细胞克隆，与野生型细胞相比，vinculin突变的细胞表现出更圆的形态和在塑料或纤维粘连蛋白上黏附和扩散的能力，通过延时视频显微镜观察到，突变细胞的黏附减少与片脂伸展减少有关。在两种检测方法中比较对照F9细胞和纽蛋白缺失细胞的运动活性。失去纽蛋白导致细胞活力增加2.4倍。这些结果表明，纽蛋白在决定细胞形状、粘附性、表面突起活性和细胞运动方面发挥着重要作用。
Vinculin, a major constituent of focal adhesions and zonula adherens junctions, is thought to be involved in linking the microfilaments to areas of cell-substrate and cell-cell contacts, To test the role of vinculin in cell adhesion and motility, we used homologous recombination to generate F9 embryonal carcinoma and embryonic stem cell clones homozygous for a disrupted vinculin gene, When compared to wild-type cells, vinculin-mutant cells displayed a rounder morphology and a reduced ability to adhere and spread on plastic or fibronectin, Decreased adhesion of the mutant cells was associated with a reduction in lamellipodial extensions, as observed by time-lapse video microscopy. The locomotive activities of control F9 and the vinculin-null cells were compared in two assays. Loss of vinculin resulted in a 2.4-fold increase in cell motility. These results demonstrate an important role for vinculin in determining cell shape, adhesion, surface protrusive activity, and cell locomotion.