Prussian blue-Au nanocomposites actuated hemin/G-quadruplexes catalysis for amplified detection of DNA, Hg2+ and adenosine triphosphate
Prussian blue-Au nanocomposites actuated hemin/G-quadruplexes catalysis for amplified detection of DNA, Hg2+ and adenosine triphosphate
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普鲁士蓝-金纳米复合材料驱动氯高铁血红素/G-四链体催化，用于 DNA、Hg2 和三磷酸腺苷的放大检测
DOI：
10.1039/c4an00897a
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发表时间：
2014
期刊：
影响因子：
4.2
通讯作者：
Zhang Xiaojun
中科院分区：
文献类型：
--
作者：
Wang Guangfeng;Chen Ling;Zhu Yanhong;He Xiuping;Xu Gang;Zhang Xiaojun
本文设计了辣根过氧化物酶模拟DNA酶（HRP-DNAzyme）和普鲁士蓝（PB）-金（Au）纳米复合物作为多功能电化学传感平台，用于DNA、Hg 2+和三磷酸腺苷（ATP）的放大检测。通过靶探针与捕获探针的偶联，在PB-Au修饰电极上形成HRP-DNAzyme的构象变化。HRP-DNA酶（红色）的氧化还原在H2 O2存在下有效进行，其中PB作为介体刺激HRP-DNA酶的生物催化功能，并启动催化循环，带来放大的信号。特异性识别的目标DNA，汞2+和ATP允许选择性安培检测的目标分子。DNA、Hg ~（2+）和ATP的检测限分别为50 nM、30 pM和3 nM。这项工作的亮点是PB-Au纳米复合材料和HRP-DNAzyme之间的催化循环被充分利用在用于多功能传感的扩增平台中。该电催化生物传感器具有较宽的线性范围、较低的检测限、良好的选择性和操作稳定性。拟议的办法提供了一个设计简单、操作方便、易于使用的通用报告制度。
In this paper, horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) and Prussian blue (PB)–gold (Au) nanocomposites were designed as versatile electrochemical sensing platforms for the amplified detection of DNA, Hg2+ and adenosine triphosphate (ATP). By the conjugation of the target probe with the capture probe, a conformational change resulted in the formation of HRP-DNAzyme on the PB–Au modified electrode. The redox of HRP-DNAzyme (red) was efficiently carried out in the presence of H2O2, in which PB acted as a mediator stimulating the biocatalytic functions of HRP-DNAzyme and actuated a catalytic cycle bringing an amplified signal. Specific recognition of the target DNA, Hg2+ and ATP allowed selective amperometric detection of the target molecule. The detection limits of DNA, Hg2+ and ATP were 50 nM, 30 pM and 3 nM, respectively. The highlight of this work is that the catalytic cycle between PB–Au nanocomposites and HRP-DNAzyme was adequately utilized in the amplification platform for versatile sensing. The novel electrocatalytic biosensor involving only one-step incubation exhibited a wide linear range, low detection limit, and satisfactory selectivity and operational stability. The proposed approach provided an ease-of-use and universal reporting system with a simple design and easy operations.