Blood borne macrophages are essential for the triggering of muscle regeneration following muscle transplant
Blood borne macrophages are essential for the triggering of muscle regeneration following muscle transplant
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DOI：
10.1016/s0960-8966(98)00111-4
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发表时间：
1999-03-01
影响因子：
2.8
通讯作者：
Parrish, E
中科院分区：
文献类型：
--
作者：
Lescaudron, L;Peltékian, E;Parrish, E
移植卫星细胞可能是遗传性肌病肌纤维重建的一种策略。然而，它的发展需要对环境信号在再生过程中的作用有很好的理解。因此，有必要确定触发和控制体内这一过程的关键事件。我们研究了巨噬细胞是否在使用来自转基因pHuDes-nls-LacZ小鼠的骨骼肌移植物的再生过程中发挥关键作用。在移植前，用巨噬细胞炎性蛋白1-β（MIP 1-β;刺激巨噬细胞浸润或血管内皮生长因子（VEGF）刺激血管生成）调节移植物。与对照组相比，用MIP 1-β和VEGF处理移植物均加速和增强单核细胞-巨噬细胞浸润和卫星细胞分化和/或增殖。此外，VEGF治疗增加了新形成的肌管的数量。当实验诱导宿主单核细胞-巨噬细胞完全耗竭时，移植物中未发生再生。我们的数据表明，存在的血源性巨噬细胞是触发骨骼肌再生的最早期事件所必需的。对肌肉损伤后巨噬细胞行为的了解，使我们能够开发未来的卫星细胞移植治疗肌营养不良症的策略。(C)1999 Elsevier Science B. V.保留所有权利。
The transplantation of satellite cells may constitute a strategy for rebuilding muscle fibres in inherited myopathies. However, its development requires a great understanding of the role of environmental signals in the regenerative process. It is therefore essential to identify the key events triggering and controlling this process in vivo. We investigated whether macrophages play a key role in the course of the regenerative process using skeletal muscle transplants from transgenic pHuDes-nls-LacZ mice. Before grafting, transplants were conditioned with macrophage inflammatory protein 1-beta (MIP 1-beta; stimulating the macrophages infiltration or vascular endothelial growth factor (VEGF) stimulating angiogenesis). Treatment of transplants with MIP 1-beta and VEGF both accelerated and augmented monocyte-macrophage infiltration and satellite cell differentiation and/or proliferation, as compared to controls. In addition, VEGF treatment enhanced the number of newly formed myotubes. When a complete depletion of host monocyte-macrophages was experimentally induced, no regeneration occurred in transplants. Our data suggest that the presence of blood borne macrophages is required for triggering the earliest events of skeletal muscle regeneration. The understanding of macrophage behaviour after muscle injury should allow us to develop future strategies of satellite cell, transplantation as a treatment for muscular dystrophies. (C) 1999 Elsevier Science B.V. All rights reserved.