HYDROLYSIS OF FMET TRANSFER RNA BY PEPTIDYL TRANSFERASE
HYDROLYSIS OF FMET TRANSFER RNA BY PEPTIDYL TRANSFERASE
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DOI：
10.1073/pnas.68.12.3163
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发表时间：
1971-01-01
影响因子：
11.1
通讯作者：
ROSMAN, M
中科院分区：
文献类型：
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作者：
CASKEY, CT;BEAUDET, AL;ROSMAN, M
大肠杆菌和兔网织红细胞（f[3 H]Met-tRNA·AUC·核糖体）中间体在丙酮存在下加入tRNA或CpCpA后发生水解，释放出f[3 H]蛋氨酸。这种核糖体催化的反应具有与肽基转移酶类似的要求、最适pH和抗生素敏感性。两种抗生素，林可霉素与E。coliribosomes和茴香霉素与网织红细胞核糖体，抑制肽键的形成和肽基转移酶的酯交换活性，但刺激f[3 H]Met-tRNA的水解。早期的研究表明肽基转移酶活性对于f（3 H）Met-tRNA的R因子依赖性水解是必不可少的。这些研究表明，肽基转移酶具有f（3 H）Met-tRNA水解的能力，因此，可能是在肽链终止过程中肽基-tRNA切割的原因。
Escherichia coliand rabbit reticulocyte (f[3H]Met-tRNA·AUG·ribosome) intermediates undergo hydrolysis, with release of f[3H]methionine, upon addition of tRNA or CpCpA in the presence of acetone. This ribosomal catalyzed reaction has similar requirements, pH optimum, and antibiotic sensitivity to those of peptidyl transferase. Two antibiotics, lincomycin withE. coliribosomes and anisomycin with reticulocyte ribosomes, inhibit peptide-bond formation and transesterification activities of peptidyl transferase, but stimulate hydrolysis of f[3H]Met-tRNA. Earlier studies have suggested peptidyl transferase activity is essential for R factor-dependent hydrolysis of f(3H)Met-tRNA. These studies indicate that peptidyl transferase has the capacity for f(3H)Met-tRNA hydrolysis and, therefore, may be responsible for peptidyl-tRNA cleavage during peptide chain termination.