DISTINCT PATTERNS OF CAMP-DEPENDENT PROTEIN-KINASE GENE-EXPRESSION IN MOUSE-BRAIN
DISTINCT PATTERNS OF CAMP-DEPENDENT PROTEIN-KINASE GENE-EXPRESSION IN MOUSE-BRAIN
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DOI：
10.1016/0896-6273(89)90116-5
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发表时间：
1989-07-01
期刊：
影响因子：
16.2
通讯作者：
MCKNIGHT, GS
中科院分区：
文献类型：
--
作者：
CADD, G;MCKNIGHT, GS
用原位杂交技术对成年小鼠中枢神经系统内cAMP依赖的蛋白激酶(PKA)mRNAs进行了定位。PKA全酶含有两个催化(C)亚基和一个调节(R)亚基二聚体。我们的研究表明，C的两个亚型(Ca和CB)和R的四个亚型(Rla、RIP、R11a和RllfI)在中枢神经系统中表达。钙、RLA和RIP的mRNAs优先定位于新皮质、尾壳核、下丘脑、丘脑和海马体。与CG和RLIG探针的杂交明显区别于类钙模式，因为与海马CAL-3细胞层相比，丘脑的杂交水平降低，齿状回的表达相对增加。R11a转录本非常特异地定位于内侧缰核。PKA亚单位基因的差异表达提示，神经组织内cAMP反应的功能差异可能是由特定的PKA亚型的生化特性所介导的。
In situ hybridization was used to localize CAMP-dependent protein kinase (PKA) mRNAs in the adult mouse CNS. The PKA holoenzyme contains two catalytic (C) subunits and a regulatory (R) subunit dimer. Our studies demonstrate expression of two isoforms of C (Ca and Cb) and four isoforms of R (Rla, RIP, Rlla, and Rllfi) in the CNS. mRNAs for Ca, Rla, and RIP preferentially localize in the neocortex, caudate-putamen, hypothalamus, thalamus, and hippocampus. Hybridization with Cg and Rllg probes is clearly distinguished from the Ca-like pattern by a reduced level of hybridization in the thalamus and by a relative increase in expression in the dentate gyrus compared with cell layers CAl-3 in the hippocampus. Rlla transcripts are very specifically localized in the medial habenula. The differential expression of PKA subunit genes suggests that functional differences in CAMP responses within neural tissues may be mediated by the biochemical properties of specific PKA isoforms.