Two-component systems of the myxobacteria: structure, diversity and evolutionary relationships
Two-component systems of the myxobacteria: structure, diversity and evolutionary relationships
复制标题
DOI：
10.1099/mic.0.2007/013672-0
复制
发表时间：
2008-02-01
期刊：
影响因子：
2.8
通讯作者：
Cock, Peter J. A.
中科院分区：
文献类型：
--
作者：
Whitworth, David E.;Cock, Peter J. A.
双组分系统（TCS）是一个大家族的信号传导途径，其特征在于磷酰基在成对组氨酸激酶和反应调节蛋白的组氨酸和天冬氨酸残基之间的连续转移。随着四个属的粘细菌的基因组序列的可用性，它已成为可能，以评估粘细菌TCS基因的基因组互补和表征其组织和进化遗产的功能。在这项研究中，我们编制了粘细菌基因组中的TCS基因的列表，并表征了它们的结构域架构，基因组织和进化关系。为了给我们的结论提供一个合适的背景，在可能的情况下，我们将粘细菌TCS与其他316种完全测序的细菌进行了比较。粘细菌是所有生物体中TCS数量最多的。一个异常低比例的TCS基因配对的粘细菌基因组中，粘细菌组氨酸激酶似乎也感觉到内部信号到一个不寻常的程度，系统发育的证据使我们能够建议同源的蛋白质的关系，在整个粘细菌，它似乎粘细菌TCS的进化一直占主导地位的复制，基因重排和变化的感觉域互补。这里提出的粘细菌的TCS蛋白的系统分类也应该提供一个框架，为未来的实验研究在这些生物体中的双组分调节。
Two-component systems (TCSs) are a large family of signalling pathways characterized by the successive transfer of phosphoryl groups between the histidine and aspartate residues of paired histidine kinase and response regulator proteins. With the availability of genome sequences for four genera of myxobacteria it has become possible to assess the genomic complements of myxobacterial TCS genes and to characterize features of their organization and evolutionary heritage. In this study we have compiled lists of the TCS genes within myxobacterial genomes and characterized their domain architecture, gene organization and evolutionary relationships. In order to provide an appropriate context for our conclusions, where possible we have compared myxobacterial TCSs with those found in 316 other completely sequenced bacteria. Myxobacteria have the largest number of TCSs of any organisms. An unusually low proportion of TCS genes are paired in myxobacterial genomes, and myxobacterial histidine kinases also seem to sense internal signals to an unusual degree, Phylogenetic evidence has allowed us to suggest homologous relationships of proteins across the myxobacteria, and it appears that myxobacterial TCS evolution has been dominated by duplications, gene rearrangements and changes in sensory domain complements. The systematic classification of the TCS proteins of the myxobacteria presented here should also provide a framework for future experimental studies on two-component regulation in these organisms.