Interrogation of N-linked oligosaccharides using infrared multiphoton dissociation in FT-ICR mass spectrometry
Interrogation of N-linked oligosaccharides using infrared multiphoton dissociation in FT-ICR mass spectrometry
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DOI：
10.1021/ac0600656
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发表时间：
2006-07-15
影响因子：
7.4
通讯作者：
Lebrilla, Carlito B.
中科院分区：
文献类型：
--
作者：
Lancaster, Katherine S.;An, Hyun Joo;Lebrilla, Carlito B.
低聚糖的结构解析仍然是一个主要的挑战。质谱法为基于串联质谱法的结构分析提供了一种快速、方便的方法。通常选择离子并进行碰撞诱导解离（CID）以获得结构信息。不幸的是，n -链低聚糖是相对较大的化合物，不容易使用CID碎片化。在这篇报告中，我们说明了使用红外多光子解离（IRMPD）来获得大n链低聚糖的结构信息。对高甘露糖型低聚糖的IRMPD和CID行为进行了比较。在傅里叶变换离子回旋共振质谱法中，观察到n -链寡糖不能通过常规CID得到破碎。比较了同样大尺寸的o -链和n -链聚糖。研究发现，只有n -链寡糖存在内部交叉环断裂。n链低聚糖的甘露糖分支点显然更容易受到交叉环断裂的影响。
The structural elucidation of oligosaccharides remains a major challenge. Mass spectrometry provides a rapid and convenient method for structural elucidation based on tandem mass spectrometry. Ions commonly are selected and subjected to collision-induced dissociation (CID) to obtain structural information. Unfortunately, N-linked oligosaccharides are relatively large compounds and are not readily fragmented using CID. In this report, we illustrate the use of infrared multiphoton dissociation (IRMPD) to obtain structural information for large N-linked oligosaccharides. The IRMPD and CID behavior of oligosaccharides were compared for high-mannose-type oligosaccharides. Fragmentation that could not be obtained through conventional CID in Fourier transform ion cyclotron resonance mass spectrometry was observed with N-linked oligosaccharides. O-Linked and N-linked glycans of similarly large sizes were compared. It was found that internal cross-ring cleavages were observed only for N-linked oligosaccharides. The mannose branch points of N-linked oligosaccharides are apparently more susceptible to cross-ring cleavages.