Electrospun aligned poly(propylene carbonate) microfibers with chitosan nanofibers as tissue engineering scaffolds
Electrospun aligned poly(propylene carbonate) microfibers with chitosan nanofibers as tissue engineering scaffolds
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DOI：
10.1016/j.carbpol.2014.10.025
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发表时间：
2015-03-06
影响因子：
11.2
通讯作者：
Turng, Lih-Sheng
中科院分区：
文献类型：
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作者：
Jing, Xin;Mi, Hao-Yang;Turng, Lih-Sheng
在这项研究中，通过静电纺丝制备了纤维直径为1.48垂直于0.42 μ m的平行排列聚碳酸亚丙酯（PPC）微纤维，并通过氧等离子体处理进行改性。接下来，通过冷冻干燥将纤维直径尺寸为278 +/-98 nm的壳聚糖纳米纤维引入PPC纤维垫中。形态学分析表明，0.05 mg/ml壳聚糖溶液处理的PPC支架具有最佳的微观结构和微纳米结构，且含有大量的壳聚糖纳米纤维，但未形成膜。用X射线光电子能谱（XPS）分析了表面化学性质。支架的初始水接触角从纯PPC支架的122.3 +/- 0.4度降低到具有等离子体处理和壳聚糖纳米纤维的支架的53.8 +/- 1.6度。等离子体处理影响支架的力学性能，杨氏模量降低63%。同时，杨氏模量经历了26%的提高后，引入壳聚糖纳米纤维。在支架上培养成纤维细胞，研究等离子体处理和引入壳聚糖纳米纤维对细胞粘附、增殖和形态的影响。与PPC微纤维和壳聚糖纳米纤维的支架显示出优于其他支架的细胞附着，细胞增殖和细胞-支架相互作用方面的上级细胞反应。(C)2014爱思唯尔有限公司版权所有。
In this study, parallel-aligned poly(propylene carbonate) (PPC) microfibers with a fiber diameter of 1.48 perpendicular to 0.42 mu m were prepared by electrospinning and modified by oxygen plasma treatment. Next, chitosan nanofibers with a fiber diameter size of 278 +/- 98 nm were introduced into the PPC fiber mats by freeze drying. Morphological analyses showed that the PPC scaffolds treated with 0.05 mg/ml chitosan solution provided the best micro and nanofiber structure with abundant chitosan nanofibers but without the formation of films. Surface chemical properties were analyzed by X-ray photoelectron spectroscopy (XPS). The initial water contact angle of the scaffolds decreased from 122.3 +/- 0.4 degrees for neat PPC scaffolds to 53.8 +/- 1.6 degrees for scaffolds with plasma treatment and chitosan nanofibers. The mechanical properties of the scaffolds were affected by plasma treatment with Young's modulus experiencing a reduction of 63%. Meanwhile, Young's modulus experienced a 26% improvement after the introduction of chitosan nanofibers. Fibroblast cells were cultured on the scaffolds to study the effects of both the plasma treatment and the introduction of chitosan nanofibers on cell adhesion, proliferation, and morphology. The scaffolds with PPC microfibers and chitosan nanofibers showed a superior cell response in terms of cell attachment, cell proliferation, and cell-scaffold interactions over the other scaffolds. (C) 2014 Elsevier Ltd. All rights reserved.