Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid
Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid
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DOI：
10.1016/j.theriogenology.2009.11.013
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发表时间：
2010-04-15
期刊：
影响因子：
2.8
通讯作者：
Nagai, T.
中科院分区：
文献类型：
--
作者：
Agung, B.;Piao, Y.;Nagai, T.
采用静态和非静态（旋转）培养系统，研究猪卵母细胞在猪卵泡液（pFF）中体外成熟过程中，氧分压和卵泡细胞（FC）对卵母细胞核成熟和体外受精的影响。在第一个实验中，使用静态（S）和旋转（R）培养系统（+FC/S、FC/S、+FC/R和FC/R），在5%或20%O-2下，在添加（+）或不添加（-）FC（5.2 x 10（6）个细胞/mL）的pFF中使卵丘-卵母细胞复合体（COC）成熟48 h。在静态培养系统（+FC/S）中与FC共培养对卵母细胞的减数分裂能力有不利影响，而在旋转培养系统（+FC/R）中与FC共培养提高成熟率。在两种培养体系中，氧分压对卵母细胞减数分裂能力没有明显影响，与培养体系和FC添加无关。在第二个试验中，COCs在5%或20%O-2下使用FC/S或+FC/R培养系统成熟，然后受精。氧张力没有显着影响受精参数，无论培养系统。与静态培养系统相比，旋转培养系统提高了精子穿透率和雄原核形成率，降低了多精受精率（P < 0.05）。总之，FC/S和+FC/R培养系统支持减数分裂能力，无论氧张力。但+FC/R培养系统在促进受精方面可能上级FC/S培养系统。(C)2010年爱思唯尔公司All rights reserved.
The objective was to investigate the effects of oxygen tension and follicle cells (FCs) during in vitro maturation of porcine oocytes in only porcine (Sus scrofa domesticus) follicular fluid (pFF), using static and non-static (rotating) culture systems, on the nuclear maturation and subsequent in vitro fertilization of the oocytes. In the first experiment, cumulus-oocyte complexes (COCs) were matured for 48 h in pFF supplemented with (+) or without (-) FCs (5.2 x 10(6) cells/mL), using the static (S) and rotating (R) culture systems (+FC/S, FC/S, +FC/R, and FC/R) under 5% or 20% O-2. Co-culture with FCs in the static culture system (+FC/S) had a detrimental effect on the meiotic competence of oocytes, whereas co-culture with FCs in the rotating culture system (+FC/R) increased maturation rates. In both culture systems, oxygen tension had no apparent effects on meiotic competence of oocytes, irrespective of culture system and FC addition. In the second experiment, COCs were matured under 5% or 20% O-2 using the FC/S or +FC/R culture systems and then fertilized. Oxygen tension had no significant effects on fertilization parameters, irrespective of the culture system. The rotating culture system increased rates of sperm penetration and male pronuclear formation and decreased polyspermic fertilization compared with the static culture system (P < 0.05). In conclusion, both FC/S and +FC/R culture systems supported meiotic competence, irrespective of oxygen tension. However, the +FC/R culture system may be superior to the FC/S culture system for promoting fertilization. (C) 2010 Elsevier Inc. All rights reserved.