Cellular mechanisms of slime gland refilling in Pacific hagfish (Eptatretus stoutii)
Cellular mechanisms of slime gland refilling in Pacific hagfish (Eptatretus stoutii)
复制标题
太平洋盲鳗（Eptatretus stoutii）粘液腺再填充的细胞机制
DOI：
10.1242/jeb.183806
复制
发表时间：
2018
影响因子：
2.8
通讯作者：
D. Fudge
中科院分区：
文献类型：
--
作者：
S. Schorno;T. Gillis;D. Fudge
抽象的鳗鱼使用它们的防御性粘液来抵御呼吸鳃的捕食者。粘液腺重新填充是一个令人惊讶的缓慢过程，之前的研究表明，在重新填充过程中，粘液渗出物的成分会发生显著变化，这可能会对粘液的功能产生影响。这项研究旨在通过研究腺体再充盈所涉及的细胞过程，以及确定腺体中主要的粘液细胞--腺线细胞和腺体粘液细胞--出现的位置，来扩展我们对粘液腺再充盈的理解。对粘液腺进行电刺激以耗尽其粘液储存，让其重新填充设定的时间段，并收获以进行组织学和免疫组织化学检查。在灌装周期中检查整个粘液腺、腺线细胞形态计量学和粘液细胞比例。粘液腺在耗尽后显著缩小，但在重新充盈后大小稳步增加。腺丝细胞是粘液腺再充盈的限制因素，其充盈和成熟所需的时间比腺粘液细胞长。新产生的腺丝细胞大部分生长在腺体边缘附近，较大的细胞距离腺体边缘最远。免疫组织化学分析还显示，仅在粘液腺的上皮衬里内有增殖细胞，这表明新的粘液细胞来自于粘液腺衬里的未分化细胞。我们的结果提供了一个深入的观察细胞动力学的粘液腺补充太平洋鳗，并提供了一个模型如何粘液腺补充在细胞水平。重点文章：鳗鱼粘液腺重新充填是一个漫长的过程；新的粘液细胞在粘液腺的上皮层中产生，并在重新充填过程中在其下方产生新细胞时移动到腺体中心。
ABSTRACT Hagfishes use their defensive slime to ward off gill-breathing predators. Slime gland refilling is a surprisingly slow process, and previous research has shown that the composition of the slime exudate changes significantly during refilling, which likely has consequences for the functionality of the slime. This study set out to expand our understanding of slime gland refilling by examining the cellular processes involved in refilling of the glands, as well as determining where in the gland the main slime cells – the gland thread cells and gland mucous cells – arise. Slime glands were electro-stimulated to exhaust their slime stores, left to refill for set periods of time, and harvested for histological and immunohistochemical examination. Whole slime glands, gland thread cell morphometrics and slime cell proportions were examined over the refilling cycle. Slime glands decreased significantly in size after exhaustion, but steadily increased in size over refilling. Gland thread cells were the limiting factor in slime gland refilling, taking longer to replenish and mature than gland mucous cells. Newly produced gland thread cells underwent most of their growth near the edge of the gland, and larger cells were found farthest from the edge of the gland. Immunohistochemical analysis also revealed proliferating cells only within the epithelial lining of the slime gland, suggesting that new slime cells originate from undifferentiated cells lining the gland. Our results provide an in-depth look at the cellular dynamics of slime gland refilling in Pacific hagfish, and provide a model for how slime glands refill at the cellular level. Highlighted Article: Hagfish slime gland refilling is a lengthy process; new slime cells are produced in the epithelial lining of the slime gland, and are moved to the center of the gland as new cells are produced below them during refilling.