The dst2 gene essential for photomorphogenesis of Coprinopsis cinerea encodes a protein with a putative FAD-binding-4 domain
The dst2 gene essential for photomorphogenesis of Coprinopsis cinerea encodes a protein with a putative FAD-binding-4 domain
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DOI：
10.1016/j.fgb.2009.10.006
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发表时间：
2010-02-01
影响因子：
3
通讯作者：
Kamada, Takashi
中科院分区：
文献类型：
--
作者：
Kuratani, Masaki;Tanaka, Kanako;Kamada, Takashi
鸡冠花子实体原基具有显着的光形态发生。在12h光照/12h黑暗条件下，原基进入子实体成熟阶段，原基依次经历担孢子的形成、柄的伸长和菌盖的扩张，最终形成成熟的子实体。然而，在连续的黑暗中，原基永远不会进入成熟期：原基上部的菌盖和柄组织仍然发育不全，而原基的基部伸长，产生黄化的“暗柄”表型。在我们之前的研究中，已经分离到在光照条件下产生暗纹的盲突变体，促进了野生型子实体的成熟，并鉴定了两个与突变表型有关的基因dst1和dst2。在本研究中，我们发现dst2-1突变体除了在子实体光形态发生过程中表现出盲性表型外，还在无性孢子产生过程中表现出盲性表型。我们还发现，dst2编码一个含有黄素腺嘌呤二核苷酸(FAD)结合-4结构域的蛋白。这两种盲性表型，以及Dst2中FAD结合域的存在，表明Dst2可能在蓝光感知中发挥作用。(C)2009 Elsevier Inc.保留所有权利。
The fruiting-body primordium of Coprinopsis cinerea exhibits remarkable photomorphogenesis. Under a 12-h light/12-h dark regime, the primordium proceeds to the fruiting-body maturation phase in which the primordium successively undergoes basidiospore formation, stipe elongation and pileus expansion, resulting in the mature fruiting-body. In continuous darkness, however, the primordium never proceeds to the maturation phase: the pileus and stipe tissues at the upper part of the primordium remain rudimentary while the basal part of the primordium elongates, producing the etiolated "dark stipe" phenotype. In our previous studies, blind mutants, which produce dark stipes under light conditions that promote fruiting-body maturation in the wild-type, have been isolated, and two genes, dst1 and dst2, responsible for the mutant phenotype have been identified. In this study we show that the dst2-1 mutant exhibits a blind phenotype during asexual spore production in addition to that in fruiting-body photomorphogenesis. We also reveal that dst2 is predicted to encode a protein with a putative flavin adenine dinucleotide (FAD)-binding-4 domain. The two blind phenotypes, together with the existence of an FAD-binding domain in Dst2, suggest that Dst2 may play a role in perceiving blue light. (c) 2009 Elsevier Inc. All rights reserved.