The intestinal microflora of childhood patients with indicated celiac disease
The intestinal microflora of childhood patients with indicated celiac disease
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DOI：
10.1007/s12223-008-0028-8
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发表时间：
2008-07-01
影响因子：
2.6
通讯作者：
Tuckova, L.
中科院分区：
文献类型：
--
作者：
Kopecny, J.;Mrazek, J.;Tuckova, L.
儿童患者的粪便短链脂肪酸浓度（154 +/- 46.9 mmol/L）高于健康儿童（96.6 +/- 19.2 mmol/L）。另一方面，患者粪便的pH值无显著性降低（6.78 +/- 0.75 vs.儿童7.42 +/- 0.74）。使用变性梯度凝胶电泳的细菌总数，乳酸杆菌和双歧杆菌的特点，在粪便样本和十二指肠活检的微生物种群。细菌粘附十二指肠活组织检查并不占主导地位的粪便样本。超过50%的检测到的细菌物种属于尚未培养的菌株。
The fecal short-chain fatty acids concentration was higher (154 +/- 46.9 mmol/L) in childhood patients than in healthy children (96.6 +/- 19.2 mmol/L). On the other hand, pH values were nonsignificantly lower in patients stool (6.78 +/- 0.75 vs. children 7.42 +/- 0.74). Using denaturing gradient gel electrophoresis specific for total bacteria, lactobacilli and bifidobacteria the microbial population was characterized in fecal samples and in duodenal biopsies. Bacteria adhering to duodenal biopsies were not dominating in stool samples. More than 50 % of detected bacterial species belonged to as yet uncultured strains.